Research on Mechanism of Exogenous Melatonin Effects on Radish Growth and Resistence to Alternaria brassicae

doi:10.16420/j.issn.0513-353x.2021-0423

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 548-560.doi: 10.16420/j.issn.0513-353x.2021-0423

• Research Papers • Previous Articles Next Articles

Research on Mechanism of Exogenous Melatonin Effects on Radish Growth and Resistence to Alternaria brassicae

XIA Ming¹^,², LI Jingwei¹^,², LUO Zhangrui², ZU Guidong³, WANG Ya⁴, ZHANG Wanping¹^,²^,^*()

¹Vegetable Research Academy,Guizhou University,Guiyang 550000,China
²College of Agriculture,Guizhou University,Guiyang 550000,China
³Agriculture and Rural Affairs Bureau of Weining County,Bijie,Guizhou 551700,China
⁴Department of Agriculture and Rural Affairs of Guizhou Province,Guiyang 550000,China

Received:2021-05-06 Revised:2021-09-09 Online:2022-03-25 Published:2022-03-25
Contact: ZHANG Wanping E-mail:1226190368@qq.com

Abstract

Abstract:

In this study,the seedlings leaves of‘Jiangnan Yuanbai’radish（Raphanus sativus）were sprayed with 0,50,100,1 000 and 1 500 μmol · L^-1 melatonin solution,the effects of exogenous melatonin on plant biomass,total chlorophyll content,APX enzyme activity,content of H₂O₂,endogenous melatonin and subcellular structures were measured,disease indexes after Alternaria brassicae inoculation were also detected. It was found that within the concentrations of 50,500 μmol · L^-1 melatonin increased growth and resistence of seedling with the increase of concentration,and 500 μmol · L^-1 treatment showed the best performance,while the indexes of 1 000 μmol · L^-1 and 1 500 μmol · L^-1 treatments decreased sharply. The results showed that the melatonin influences radish growth and resistence relying on a dose-dependent effect.RNA-Seq based transcriptome analysis was conducted on 0,500 and 1 500 μmol · L^-1melatonin treated samples,eight key DEGs were selected,which were Ubr,acting on photosynthetic regulation,and Aos1,Prp1,Zat-like,Jub1-like,Edr1,Usp and Eda,regulating resistence. The expression pattern of DEGs was consistent with the phenotypic changes of all treatments,showed a dose-dependent pattern.

Key words: Raphanus sativus, melatonin, Alternaria brassicae, resistence, transcriptome

CLC Number:

S631.1

XIA Ming, LI Jingwei, LUO Zhangrui, ZU Guidong, WANG Ya, ZHANG Wanping. Research on Mechanism of Exogenous Melatonin Effects on Radish Growth and Resistence to Alternaria brassicae[J]. Acta Horticulturae Sinica, 2022, 49(3): 548-560.

Figures/Tables 9

References 55

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基因名 Gene ID	基因名 Gene name	描述 Description	KEGG通路ID KEGG pathway ID	引物序列（5′-3′） Primer sequence
108828933	Aos1	丙二烯氧化物合酶,叶绿体 Allene oxide synthase,chloroplastic	Ko00592：α-亚麻酸代谢 α-Linolenic acid metabolism	F：ACCTACATGCCCTCGACGGATC R：TTGGCGTGTTTCGGCTCTGATG
108859130	Prp1	致病相关类蛋白1 Pathogenesis-related protein 1-like	Ko04626：植物—病菌互作 Plant-pathogen interaction	F：AACCAAGCACGACAGGCAGTAAG R：ACTCTTAGTCGGTCGGCGTAGC
108818819	At-like	锌指类蛋白ZAT10 Zinc finger protein ZAT10-like	无Naught	F：TCCACCACCAGAAGAACCTCACC R：GCACTTGTAGGACGACTTCTCAGC
108834050	Jub1-like	JUNGBRUNNEN类转录因子1 Transcription factor JUNGBRUNNEN 1-like	Ko04141：内质网中的蛋白质加工Protein processing in endoplasmic reticulum	F：CACAAGCCACACGAGCCACAG R：AATCTCCACCGTCGAGGTTCCC
108862765	Edr1	丝氨酸/苏氨酸蛋白激酶EDR1 Serine/threonine-protein kinase EDR1	无Naught	F：TTCGCAGACCTTAACCCGTTTCAG R：TGCAGGAGACTTACCAACCAGAGG
108848467	Ubr	紫外线B受体UVR8亚型X1 Ultraviolet-B receptor UVR8 isoform X1	Ko04120：泛素介导的蛋白质水解 Ubiquitin mediated proteolysis	F：AGTCACCGTTCTCCTCCTTCTCG R：TTGTTGCGTGGACTGCTCGATTAG
108860024	Usp	普遍应激蛋白A样蛋白 Universal stress protein A-like protein	无Naught	F：ACGAAAGCGAAGAGAGCATGGAAG R：CCCTGCGGTGTCAATGGAAGAG
108807199	Ech-like	类内源几丁质酶At2g43590 Endochitinase At2g43590-like	Ko00520：氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	F：CCTCGGGCGTATGTTGTAGTCAG R：GCAAGGACCTGATCGGCAACC
108847559	Actin-1 Actin-2	actin-7-like actin-7-like	无Naught 无Naught	F：GCATCACACTTTCTACAAC R：CCTGGATAGCAACATACAT F：GCAAGAGCTGGATACCGCAAAG R：CGATGAGCGATGGCTGGAAC

基因名 Gene ID	基因名 Gene name	描述 Description	KEGG通路ID KEGG pathway ID	引物序列（5′-3′） Primer sequence
108828933	Aos1	丙二烯氧化物合酶,叶绿体 Allene oxide synthase,chloroplastic	Ko00592：α-亚麻酸代谢 α-Linolenic acid metabolism	F：ACCTACATGCCCTCGACGGATC R：TTGGCGTGTTTCGGCTCTGATG
108859130	Prp1	致病相关类蛋白1 Pathogenesis-related protein 1-like	Ko04626：植物—病菌互作 Plant-pathogen interaction	F：AACCAAGCACGACAGGCAGTAAG R：ACTCTTAGTCGGTCGGCGTAGC
108818819	At-like	锌指类蛋白ZAT10 Zinc finger protein ZAT10-like	无Naught	F：TCCACCACCAGAAGAACCTCACC R：GCACTTGTAGGACGACTTCTCAGC
108834050	Jub1-like	JUNGBRUNNEN类转录因子1 Transcription factor JUNGBRUNNEN 1-like	Ko04141：内质网中的蛋白质加工Protein processing in endoplasmic reticulum	F：CACAAGCCACACGAGCCACAG R：AATCTCCACCGTCGAGGTTCCC
108862765	Edr1	丝氨酸/苏氨酸蛋白激酶EDR1 Serine/threonine-protein kinase EDR1	无Naught	F：TTCGCAGACCTTAACCCGTTTCAG R：TGCAGGAGACTTACCAACCAGAGG
108848467	Ubr	紫外线B受体UVR8亚型X1 Ultraviolet-B receptor UVR8 isoform X1	Ko04120：泛素介导的蛋白质水解 Ubiquitin mediated proteolysis	F：AGTCACCGTTCTCCTCCTTCTCG R：TTGTTGCGTGGACTGCTCGATTAG
108860024	Usp	普遍应激蛋白A样蛋白 Universal stress protein A-like protein	无Naught	F：ACGAAAGCGAAGAGAGCATGGAAG R：CCCTGCGGTGTCAATGGAAGAG
108807199	Ech-like	类内源几丁质酶At2g43590 Endochitinase At2g43590-like	Ko00520：氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	F：CCTCGGGCGTATGTTGTAGTCAG R：GCAAGGACCTGATCGGCAACC
108847559	Actin-1 Actin-2	actin-7-like actin-7-like	无Naught 无Naught	F：GCATCACACTTTCTACAAC R：CCTGGATAGCAACATACAT F：GCAAGAGCTGGATACCGCAAAG R：CGATGAGCGATGGCTGGAAC

褪黑素/（μmol · L^-1） Melatonin	株高/cm Plant height	茎粗/mm Stem diameter	鲜质量/g Fresh weight	干质量/g Dry weight
ddH₂O（对照Control）	13.57 ± 0.19 d	3.37 ± 0.04 cd	126.33 ± 0.93 d	10.75 ± 0.05 d
50	14.47 ± 0.18 c	3.73 ± 0.03 b	130.07 ± 0.87 c	11.81 ± 0.08 c
100	15.33 ± 0.22 b	3.90 ± 0.03 ab	139.17 ± 0.83 b	12.34 ± 0.06 b
500	16.11 ± 0.10 a	4.05 ± 0.07 a	156.27 ± 0.83 a	12.76 ± 0.04 a
1 000	14.79 ± 0.14 c	3.52 ± 0.05 c	126.53 ± 0.45 d	10.78 ± 0.16 d
1 500	12.91 ± 0.08 e	3.29 ± 0.03 d	123.61 ± 0.66 e	10.41 ± 0.10 e

褪黑素/（μmol · L^-1） Melatonin	株高/cm Plant height	茎粗/mm Stem diameter	鲜质量/g Fresh weight	干质量/g Dry weight
ddH₂O（对照Control）	13.57 ± 0.19 d	3.37 ± 0.04 cd	126.33 ± 0.93 d	10.75 ± 0.05 d
50	14.47 ± 0.18 c	3.73 ± 0.03 b	130.07 ± 0.87 c	11.81 ± 0.08 c
100	15.33 ± 0.22 b	3.90 ± 0.03 ab	139.17 ± 0.83 b	12.34 ± 0.06 b
500	16.11 ± 0.10 a	4.05 ± 0.07 a	156.27 ± 0.83 a	12.76 ± 0.04 a
1 000	14.79 ± 0.14 c	3.52 ± 0.05 c	126.53 ± 0.45 d	10.78 ± 0.16 d
1 500	12.91 ± 0.08 e	3.29 ± 0.03 d	123.61 ± 0.66 e	10.41 ± 0.10 e

褪黑素/（μmol · L^-1） Melatonin	叶片褪黑素含量/（ng · mL^-1） Melatonin content of leaf	SPAD	H₂O₂含量/（mmol · g^-1 FW） H₂O₂ content	APX活性/（μmol · g^-1 FW） APX activity
ddH₂O（对照Control）	14.36 ± 1.12 c	32.66 ± 0.54 d	7.35 ± 0.12 a	0.372 ± 0.009 e
50	—	34.98 ± 0.24 c	6.37 ± 0.08 b	0.551 ± 0.037 d
100	—	36.94 ± 0.23 b	5.64 ± 0.08 c	0.752 ± 0.017 c
500	19.23 ± 0.60 b	38.49 ± 0.27 a	4.69 ± 0.17 d	1.070 ± 0.023 a
1 000	—	35.58 ± 0.06 c	5.73 ± 0.06 c	0.863 ± 0.034 b
1 500	25.04 ± 0.82 a	32.79 ± 0.63 d	6.51 ± 0.14 b	0.422 ± 0.005 e

Research on Mechanism of Exogenous Melatonin Effects on Radish Growth and Resistence to Alternaria brassicae

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基因ID Gene ID	基因名 Gene name	褪黑素处理（μmol · L^-1） Melatonin treatment			Log₂比值 Log₂Ratio
基因ID Gene ID	基因名 Gene name	ddH₂O	500	1 500	500 vs ddH₂O	1 500 vs ddH₂O	500 vs 1 500
108828933	Aos1	6.04	37.51	4.12	6.21	0.68	9.10
108859130	Prp1	12.48	65.54	29.11	5.25	2.33	2.25
108818819	Zat-like	17.79	41.10	18.44	2.30	1.04	2.23
108834050	Jub1-like	0.05	0.65	0.44	13.00	8.80	1.47
108862765	Edr1	6.34	6.70	4.18	1.06	0.65	1.60
108848467	Ubr	2.65	3.88	2.13	1.46	0.80	1.82
108860024	Usp	180.23	268.96	9.09	1.49	0.05	29.58
108807199	Ech-like	251.88	670.56	5.13	2.66	0.02	130.71

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