Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 62-72.doi: 10.16420/j.issn.0513-353x.2020-1058

• Research Papers • Previous Articles     Next Articles

Transcriptome Analysis of Waxy Near-isogenic Lines in Chinese Cabbage Floral Axis

WANG Ronghua, WANG Shubin, LIU Shuantao, LI Qiaoyun, ZHANG Zhigang, WANG Lihua, ZHAO Zhizhong()   

  1. Vegetable and Flower Institute,Shandong Academy of Agricultural Sciences,Shandong Branch of National Center for Vegetable Improvement,Shandong Key Laboratory for Biology of Greenhouse Vegetables,Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables,Ministry of Agriculture and Rural Affairs(Shandong),Ji’nan 250100,China
  • Received:2021-03-01 Revised:2021-08-19 Online:2022-01-25 Published:2022-01-24


In order to study the molecular mechanism of floral axis waxiness in Chinese cabbage,a comparative transcriptome analysis was carried out on a pair of near isogenic lines with difference in floral axis waxiness. In total,7 237 differentially expressed genes(DEGs)were identified between waxy material (RHL065_1)and non-waxy material(RHL065_2),mainly enriched in carbohydrate biological processes,cellular components of plasma membrane and tetrapyrrole binding molecular functions. Combined with gene function annotation,17 DEGs,involved in wax synthesis MAH1,CYTB5-B (Bra022898,Bra021809),KCS6/CER6/CUT1,KCS9,FAR3/CER4,KCS1,CER2,KCD/PAS2,CYTB5-C(Bra039268,Bra004518),CER2-LIKE,wax transport LTPG1(Bra010912,Bra030067)and transcription factors WIN1/SHINE1,MYB30,DEWAX were proposed to contribute to surface waxiness formation in Chinese cabbage floral axis. A further qRT-PCR analysis was used to verify the transcriptome analysis and survey the expression pattern of DEGs. These results will be helpful for further gene cloning,developing functional molecular markers and understanding the genetic architecture for waxiness.

Key words: Chinese cabbage, waxy, transcriptome, candidate gene

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