Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 148-156.doi: 10.16420/j.issn.0513-353x.2020-1018

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Methylation Analysis of Mycelium and Sclerotium Genome in Pleurotus tuber-regium

WU Junyue, SUN Xueyan, YANG Zhenhua, LUO Lu, MO Cuiyuan, MA Aimin()   

  1. College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-05-08 Revised:2021-11-08 Online:2022-01-25 Published:2022-01-24
  • Contact: MA Aimin


The mycelium and sclerotium of Pleurotus tuber-regium(Fr.)Singer were used as materials,the methylation levels were analyzed by MSAP technology,and the differentially methylated fragments were recovered and sequenced to explore the relationship between the growth of P. tuber-regium and DNA whole genome methylation.The results showed that the DNA methylation rates of mycelium and sclerotium were 7.94% and 9.54%,respectively;the hemi-methylation rates were 4.50% and 4.09%,and the fully methylation rates were 3.44% and 5.45%,respectively.That is,the formation of sclerotium was accompanied by an increase in the methylation level,the full methylation level was more representative of the overall methylation status.Partially methylated differential fragments were recovered,cloned and sequenced.The NCBI homology comparison showed that the homologous genes or proteins are mainly related to metabolism,cell growth,and stress resistance.It is speculated that P. tuber-regium might regulate the expression of these genes or proteins through methylation to form sclerotium to adapt to environmental changes.

Key words: Pleurotus tuber-regium, mycelium, sclerotium, DNA methylation, methylation-sensitive amplified polymorphism(MSAP)

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