Function Analysis of MdRGL3a Gene in Malus × domestica

doi:10.16420/j.issn.0513-353x.2016-0711

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (6): 1021-1028.doi: 10.16420/j.issn.0513-353x.2016-0711

• Research Papers • Next Articles

Function Analysis of MdRGL3a Gene in Malus × domestica

REN Yiran，ZHAO Qiang，YOU Chunxiang，and HAO Yujin*

（College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，National Research Center for Apple Engineering and Technology，Tai’an，Shandong 271018，China）

Online:2017-06-25 Published:2017-06-25

Abstract

Abstract:

In order to further reveal the mechanism of GA signal transduction in apple（Malus × domestica） and the physiological function of MdDELLAs，a gene named MdRGL3a（GenBank：DQ007887）cloned from‘Royal Gala’was analyzed. The function of this gene was analyzed by ectopic expression of MdRGL3a in Arabidopsis thaliana. Real-time quantitative PCR results showed that MdRGL3a was expressed in different tissues，and the expression level was higher in flowers and fruits. Ectopic expression of MdRGL3a in Arabidopsis thaliana reduced response to GA3，which exhibited shorter root and hypocotyl，slower germination，smaller leaves and shorter stalk. The result of Arabidopsis thaliana plants treated with GA3 showed that MdRGL3a reduced the promotion of GA for plants growth and development，revealing that MdRGL3a might be a negative regulator of GA signaling pathway.

Key words: apple, MdRGL3a, DELLA protein, expression analysis, functional identification

CLC Number:

S 661.1

REN Yiran，ZHAO Qiang，YOU Chunxiang，and HAO Yujin*. Function Analysis of MdRGL3a Gene in Malus × domestica[J]. ACTA HORTICULTURAE SINICA, 2017, 44(6): 1021-1028.

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Function Analysis of MdRGL3a Gene in Malus × domestica

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