Cloning and Function Identification of Apple Alternaria Blotch Resistant Gene Mal d 1

doi:10.16420/j.issn.0513-353x.2016-0654

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (2): 343-354.doi: 10.16420/j.issn.0513-353x.2016-0654

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Cloning and Function Identification of Apple Alternaria Blotch Resistant Gene Mal d 1

ZONG Zeran，TIAN Yi，ZHANG Liyi，HAN Xiaolei，ZHANG Caixia^*，and CONG Peihua^*

（Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（Germplasm Resources Utilization），Ministry of Agriculture，P. R. China，Research Institute of Pomology，Chinese Academy of Agricultural Sciences，Xingcheng，Liaoning 125100，China）

Online:2017-02-25 Published:2017-02-25

Abstract

Abstract:

The Mal d 1 gene，which might be involved in the Alternaria blotch resistance in apple，was amplified and sequenced using the total RNA of apple leaves as template. Sequence analyses showed that it was 480 bp in length，contained two exons，and was characterized by bet v 1-like domain. The expressions of Mal d 1 were detected，but varied in apple leaves，flowers，fruit peels and pulps based on qRT-PCR analyses. Along with the development of fruits，it expression in fruit peels was increased first and then decreased. In the inoculated leaves with Alternaria alternata，Mal d 1 was significantly upregulated compared to the control counterpart，indicating is potential role in the resistance against Alternaria blotch. Furthermore，overexpression of Mal d 1 in transgenic callus，which was obtained using PRI101-AN as the expression vector by Agrobacterium-mediated transformation method，could greatly enhance the resistance to Alternaria blotch.

Key words: apple, Mal d 1, expression analysis, transgene, Alternaria alternata apple pathotype

CLC Number:

S 661.1

ZONG Zeran，TIAN Yi，ZHANG Liyi，HAN Xiaolei，ZHANG Caixia*，and CONG Peihua*. Cloning and Function Identification of Apple Alternaria Blotch Resistant Gene Mal d 1 [J]. ACTA HORTICULTURAE SINICA, 2017, 44(2): 343-354.

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Cloning and Function Identification of Apple Alternaria Blotch Resistant Gene Mal d 1

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