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ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (12): 2446-2454.

• Ornamental Plants • Previous Articles     Next Articles

Cloning of the lilyABC1 Protein Kinase Gene and Its Expression Patterns Under Abiotic Stresses in Lilium regale

YUAN Ying-Ying-*, LIANG Yun-*, YUAN Su-Xia, XU Lei-Feng, LI Ya-Nan, Younes Pourbeyrami Hir, LIU Chun, MING Jun   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2014-12-25 Published:2014-12-25

Abstract: LilyABC1,a gene encoding protein kinase,was cloned and its expression patterns in Lilium regale Wilson under abiotic stresses were dissected for the purpose of providing a candidate gene for lily breeding for resistance to various abiotic stresses. Plant material of wild Lilium regale Wilson was used to clone the full-length cDNA sequence of lilyABC1 using RACE technology in combination with cDNA library. A number of appropriate bioinformatics softwares were used to assay the structure of cDNA sequence and the function of deduced lilyABC1. The real-time quantitative PCR(qRT-PCR)method was applied to determine lilyABC1 expression patterns in specific tissues under different abiotic stresses including PEG-6000,NaCl,low temperature,high temperature and darkness. The results showed that thelilyABC1 cDNA was 2 333 bp with a 2 007 bp open readings frame in full-length. The sequence of the lilyABC1 consists of 668 amino acids with a molecular mass of 74.84 kD. The pI value of this protein is 5.46 and containing STYKc conserved domain. The expression levels of lilyABC1 were ranked as leaf > flower bud > bulbs;There was no obvious expression difference among different development stages of the flower buds. The expression of LilyABC1 was up-regulated by NaCl,low temperature,high temperature and darkness,but it was not sensitive to the PEG-6000. Therefore,lilyABC1 might have contributed to its resistance to abiotic stresses such as NaCl,low temperature,high temperature,dark and drought.

Key words: Lilium regale, lilyABC1, gene clone, expression, abiotic stress

CLC Number: