Preliminary Studies on the Possible Mechanism Underlying 5-aminolevulinic Acid-induced Stomatal Opening in Apple Leaves

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (10): 1965-1974.

Preliminary Studies on the Possible Mechanism Underlying 5-aminolevulinic Acid-induced Stomatal Opening in Apple Leaves

CHEN Ling-hui，LIU Long-bo，AN Yu-yan，ZHANG Zhi-ping，and WANG Liang-ju*

College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China

Received:2014-06-03 Online:2014-10-25 Published:2014-10-25

Abstract

Abstract: A possible mechanism for 5-aminolevulinic acid（5-ALA）to promote stomatal aperture was studied with abaxial epidermis of detached leaves of apple（Malus ´ domenstica Korkh.‘Fuji’）combined with pharmacology，microscopy and laser-scanning confocal microscopy. It was found that 5-ALA promoted stomata opening under both light and dark conditions. It also inhibited the stomata closure induced by exogenous ABA and H2O2，and reversed the closure by Ca2+. Furthermore，5-ALA treatment was found to reduce the endogenous H2O2 and Ca2+ content in guard cells of apple leaves. Therefore，it can be deduced that 5-ALA induced stomata opening may be the result of down-regulation of H2O2 and Ca2+ content in the guard cells of apple leaves.

Key words: apple, ABA, 5-ALA, guard cells, regulatory mechanism, stomatal aperture

CLC Number:

S 661.1

Chen-Ling-Hui, LIU Long-Bo, AN Yu-Yan, ZHANG Zhi-Ping, WANG Liang-Ju. Preliminary Studies on the Possible Mechanism Underlying 5-aminolevulinic Acid-induced Stomatal Opening in Apple Leaves[J]. ACTA HORTICULTURAE SINICA, 2014, 41(10): 1965-1974.

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Preliminary Studies on the Possible Mechanism Underlying 5-aminolevulinic Acid-induced Stomatal Opening in Apple Leaves

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