Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 53-64.doi: 10.16420/j.issn.0513-353x.2021-1086

• Research Papers • Previous Articles     Next Articles

Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit

YUAN Xin1, XU Yunhe1, ZHANG Yupei1, SHAN Nan1, CHEN Chuying1, WAN Chunpeng1, KAI Wenbin1, ZHAI Xiawan1, CHEN Jinyin1,2,*(), GAN Zengyu1,*()   

  1. 1Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables,College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China
    2College of Materials and Chemical Engineering,Pingxiang University,Pingxiang,Jiangxi 330075,China
  • Received:2022-09-16 Revised:2022-11-30 Online:2023-01-25 Published:2023-01-18
  • Contact: *(


The effect of ABA on IAA metabolism during postharvest ripening of‘Hongyang’kiwifruit were studied. The results showed that exogenous ABA treatment could accelerate the degradation of free IAA,increase the content of IAA-Asp,and significantly promote the expression of IAA degradation related gene AcGH3.1. Five ABA-responsive binding factor genes(AcAREB1-AcAREB5)were isolated and identified from kiwifruit genome database. qRT-PCR showed that all five AcAREB genes could respond to ABA during postharvest ripening. Yeast one-hybrid results showed that AcAREB1 could directly bind to the AcGH3.1 gene promoter. Subcellular localization assay showed that AcAREB1 was located on the nucleus. Yeast self-activation experiments proved that AcAREB1 was a transcription factor with transcriptional activation activity. These results indicate that AcAREB1 can regulate the expression of AcGH3.1 gene corresponding to ABA during the ripening process of kiwifruit,thereby promoting the degradation of IAA,and finally leading to fruit softening and ripening.

Key words: kiwifruit, postharvest ripening, AcAREB1, AcGH3.1, promoter, ABA, IAA

CLC Number: