Molecular Clonging and Expression Analysis of Homological Gene APX from Phalaenopsis

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (4): 769-776.

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Molecular Clonging and Expression Analysis of Homological Gene APX from Phalaenopsis

XU Chuan-jun1,2，SUN Xu-zhuo2，LI Ling2,*，RU Zhi-wei2，ZENG Bi-yu1，LIU Yu-mei3，and HUANG Jun-mei1

（1 Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant，Fujian Institute of Subtropical Botany，Xiamen，Fujian 316006，China；2 Guangdong Key Lab of Biotechnology for Plant Development，College of Life Science，South China Normal University，Guangzhou 510631，China；3Xiamen Overseas Chinese Subtropical Plant IntroductionGarden，Xiamen，Fujian 361002，China）

Online:2012-04-25 Published:2012-04-25

Abstract

Abstract: The APX homolog sequence，PhAPX（GenBank accession No. FJ161977）was cloned from Phalaenopsis plant. The full length cDNA of PhAPX was 1 320 bp，has an open read frame of 747 bp，encoding a protein of 249 amino acids. Bioinformatic analysis showed that PhAPX protein shared the characters of ClassⅠof peroxidase family. Amino acids sequence analysis suggested that PhAPX protein might locate in cytoplast and PhAPX was highly similar to other APX proteins. Real-time PCR analysis showed that PhAPX mRNA，a broad-spectrum expression gene，was expressed in the organs in Phalaenopsis including root，stem，leaf and flower. PhAPX was also found to be up-regulated by wound and NaCl，which suggested that it might play a role on stress.

Key words: Phalaenopsis, APX, clone, expression, real-time PCR

CLC Number:

S 682.31

XU Chuan-Jun, SUN Xu-Zhuo, LI Ling, RU Zhi-Wei, ZENG Bi-Yu, LIU Yu-Mei, HUANG Jun-Mei. Molecular Clonging and Expression Analysis of Homological Gene APX from Phalaenopsis[J]. ACTA HORTICULTURAE SINICA, 2012, 39(4): 769-776.

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Molecular Clonging and Expression Analysis of Homological Gene APX from Phalaenopsis

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