Abstract: Based on an orthogonal design and one-way experiment，an SSR molecular marker analysis protocol was established in Lycoris chinensis for the development and utilization of Lycoris resources and marker-assisted selection，which had a total volume of 20 μL containing 0.5 U of Taq DNA polymerase，1.5 mmol · L-1 Mg 2+ ，0.125 mmol · L-1 dNTP，0.5 mmol · L-1 primer and 50 ng of template DNA. This protocol had been applied to an analysis of 8 species of Lycoris and 35 clones of L. chinensis with 7 and 9.5 polymorphic loci per primer on average obtained，respectively，which reflects a good performance in universality and stability of this protocol. Analysis of the corresponding relation between SSR loci and such traits as scape height and flowering time had preliminarily shown that loci Ly 97 and Ly 98 were correlated with late flowering time‘Oct’as well as that loci Ly 84，Ly 87 and Ly 97 with scape heights ‘Mid’and‘Low’. It is suggested to verify the reliability of these markers to be used in assisted selection by further hybridization and genetic test.

Key words: Lycoris Herb., Lycoris chinensis, SSR, correspondence analysis