Cloning and Expression Analysis of Lipoxygenase-1 Gene Encoding a Key Enzyme of the Biosynthesis of Jasmonic Acid from Gladiolus hybridus

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (3): 563-570.

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Cloning and Expression Analysis of Lipoxygenase-1 Gene Encoding a Key Enzyme of the Biosynthesis of Jasmonic Acid from Gladiolus hybridus

LIAN Qing-long，XIN Hai-bo，ZHANG Zi-you，ZHONG Xiong-hui，LUO Xian，and YI Ming-fang*

Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193，China

Received:2010-08-11 Revised:2011-01-11 Online:2011-03-25 Published:2011-03-25
Contact: YI Ming-fang

Abstract

Abstract: A full length cDNA named GhLOX1 belonging to 9-LOX was cloned in Gladiolus hybridus‘Rose Supreme’corms by RT-PCR and RACE. The open reading frame encompassed 2 541 bp encoding a polypeptide of 846 amino acids with calculated protein molecular mass of 94.90 kD. RT-PCR analysis showed that GhLOX1 gene was expressed in leaf，flower，root，stolon，corm，cormel，and higher in the leaf and stolon. In vitro，with the different concentration of salicylic acid（SA）0，0.1，0.5，1.0，2.0 mmol · L-1 treatment respectively，the expression level decreased as the concentration increased. LOX1 wasn’t expressed after treating 2.0 mmol · L-1 SA.

Key words: Gladiolus hybridus, lipoxygenase, jasmonic acid, cloning, expression

CLC Number:

S 682.2

LIAN Qing-long;XIN Hai-bo;ZHANG Zi-you;ZHONG Xiong-hui;LUO Xian;and YI Ming-fang . Cloning and Expression Analysis of Lipoxygenase-1 Gene Encoding a Key Enzyme of the Biosynthesis of Jasmonic Acid from Gladiolus hybridus [J]. ACTA HORTICULTURAE SINICA, 2011, 38(3): 563-570.

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Cloning and Expression Analysis of Lipoxygenase-1 Gene Encoding a Key Enzyme of the Biosynthesis of Jasmonic Acid from Gladiolus hybridus

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