Molecular Cloning and Expression of Flower ing-related MADS-box Gene inDiospyros kaki

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (1): 39-42.

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Molecular Cloning and Expression of Flower ing-related MADS-box Gene inDiospyros kaki

DING Yan;HAN Zhen-hai;XU Xue-feng; LI Tian-zhong^*

( Institute of Horticultural Plants, China Agricultural University, Beijing 100094, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract: A MADS-box gene named DkMADS1 was cloned from Yangfeng persimmon flowers (GenBank
accession No. DQ412058). The sequence of complete DkMADS1 cDNA gene is 1 193 bp, with an ORF en
coding 249 amino acids. The deduced protein contained conservative MADS domain and semi-conservative K
domain. It shared highly homology to other MADS-box proteins from different species. RT-PCR analysis
showed that DkMADS1 gene was exp ressed in sepal, petal, ovary of Yangfeng persimmon and stamen of Zenji
maru persimmon.

Key words: Persimmon, Diospyros kaki, Flower, Gene, Cloning, Expression

DING Yan;HAN Zhen-hai;XU Xue-feng;LI Tian-zhong. Molecular Cloning and Expression of Flower ing-related MADS-box Gene inDiospyros kaki[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 39-42.

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Molecular Cloning and Expression of Flower ing-related MADS-box Gene inDiospyros kaki

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