Establishment of Agrobacterium-mediated Transformation System of Lily

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 664-666.

• 研究简报 • Previous Articles Next Articles

Establishment of Agrobacterium-mediated Transformation System of Lily

Wang Aiju¹;Zhang Fengmei¹;Lu Jinying¹;Zhao Xiangyun²;Jia Xu¹;Liu Min^1*

( ¹ Key Laboratory of Plant Biotechnology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Beijing 100101, China; ²Department of Gardens, Beijing Agricultural College, Beijing 102206, China)

Received:2005-06-22 Revised:2005-08-30 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract: We established genetic transformation system of lily callus (Lilium‘Star Gazer’) and leaves (Lilium‘Siberia’) mediated by Agrobacterium tumefaciens. Experimental result indicates that we could get efficient transformation with both callus and leaves as accep tor. We gained high transformation rate when the OD600 of Agrobacterium was between 0.5 - 1.0. There were better screening to callus and leaveswhen the concentration of Hygromycin was 40 mg·L ^{- 1}. RemovingNH₄NO₃ from major element could remarkably enhance
transformation rate. Results of PCR proved that the gus gene had integrated into lily genome.

Key words: Lilium, Agrobacterium tumefaciens, Genetic transformation, PCR

Wang Aiju;Zhang Fengmei;Lu Jinying;Zhao Xiangyun;Jia Xu;Liu Min. Establishment of Agrobacterium-mediated Transformation System of Lily[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 664-666.

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Establishment of Agrobacterium-mediated Transformation System of Lily

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