Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 663-670.doi: 10.16420/j.issn.0513-353x.2020-1027

• New Technology and New Mathod • Previous Articles     Next Articles

TaqMan MGB-based Real-time Fluorescence PCR Method for the Rapid Detection of Ciborinia camelliae

LÜ Yan1,2, DUAN Weijun1,3,*()   

  1. 1Ningbo Academy of Inspection and Quarantine,Ningbo,Zhejiang 315012,China
    2Ningbo Joysun Product Testing Service Company,Ningbo,Zhejiang 315012,China
    3Ningbo Customs District P. R. China,Ningbo,Zhejiang 315012,China
  • Received:2021-06-07 Revised:2021-11-26 Online:2022-03-25 Published:2022-03-25
  • Contact: DUAN Weijun


Based on β-tubulin gene of Ciborinia camelliae L. M. Kohn and related isolates,a pair of specific primers and a TaqMan MGB probe were designed and synthesized. A novel real-time fluorescent PCR was established to detect C. camelliae. The optimized primer and probe concentrations were 0.8 and 0.7 μmol · L-1,respectively. The minimum detection limit of this method is up to 1.0 pg for 10 μL reaction system. The method was specific,sensitive,easily to operate and fast,which provide a valuable tool for early rapid detection and identification of C. camelliae.

Key words: Camellia, camellia flower blight, Ciborinia camelliae, TaqMan MGB probe, real-time fluorescent PCR, rapid detection

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