Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 908-920.doi: 10.16420/j.issn.0513-353x.2020-0579

• Research Papers • Previous Articles     Next Articles

The Activity Verification of pri-miR319a Encode Regulatory Peptide of Dimocarpus longan

SU Liyao1, WANG Peiyu2, JIANG Mengqi1, HUANG Shuqi1, XUE Xiaodong1, LIU Mengyu1, XIAO Xuechen1, LAI Chunwang1, ZHANG Zihao1, CHEN Yukun1, LAI Zhongxiong1, LIN Yuling1,*()   

  1. 1Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2Sanming Academy of Agricultural Sciences,Sanming,Fujian 365000,China
  • Received:2020-09-15 Revised:2021-04-06 Online:2021-05-25 Published:2021-06-07
  • Contact: LIN Yuling


To study the potential activity of pri-miR319a encoding regulatory peptide of Dimocarpus longan,the pri-miR319a full-length sequences were cloned from seven longan varieties and the positions of potential miPEP(miRNA encode regulatory peptide)in longan pri-miR319a sequences were further analyzed. Then,the activity of miPEP319a was verified by genetic transformation and artificial synthesis of miPEP319a. The results showed that among the seven longan varieties,pri-miR319a had 10 nucleotide differences and three potential ORFs that could encode miPEPs,and one nucleotide mutation site resulted in the change of miPEP319a amino acid sequence. The biological activity of longan miPEP was further verified by thein plantatransformation technology and the synthetic miPEP319 treated embryogenic callus of longan. The results indicated that only the miPEP319a-2 of the three potential miPEPs was biological activity and promoted the expression of miR319a. Finally,the vectors were transiently expressed in tobacco leaves by Agrobacterium-mediated transformation method to verify the activity of longan miPEP319a in tobacco. The results showed that there was no significant difference in the expression of miR319a in tobacco leaves,which indicated that miPEP319a was specie-specific. The results suggested that longan miPEP319a-2 had biological activity and species-specific,and also suggested that longan miPEP319a-2 might participate in the growth and development of longan.

Key words: Dimocarpus longan, miR319a, miRNA encode regulatory peptide, gene clone, genetic transformation

CLC Number: