Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1619-1625.doi: 10.16420/j.issn.0513-353x.2020-0967

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Effect of Creeping Bentgrass Small Heat Shock Protein AsHSP26.8a on Transgenic Photosynthesis in Arabidopsis thaliana

ZHU Junfei, LI Xin, DONG Kangting, TANG Zhifei, BIAN Xiuju, WANG Lihong, LI Huibin(), SUN Xinbo()   

  1. Key Laboratory of Crop Growth Regulation of Hebei Province,College of Agronomy,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2020-11-09 Revised:2021-02-09 Online:2021-08-25 Published:2021-09-06
  • Contact: LI Huibin,SUN Xinbo;


In this paper,effects of a creeping bentgrass small heat shock protein,AsHSP26.8a,on photosynthesis in transgenic Arabidopsis thaliana were studied by measuring root length,plant weight,transpiration rate,stomatal conductance,net photosynthetic rate and PSⅡlight energy conversion efficiency of AsHSP26.8 transgenic A. thaliana. Furthermore,the expression of endogenous photosynthesis-related genes(rbcL,psaA,psbA,psaE,clpP,rpoB,rpoA,accD)in A. thaliana were analyzed by qRT-PCR to reveal the regulation pathway of AsHSP26.8a in plant photosynthesis. Results showed that compared with the wild type,the root length of transgenic A. thaliana was significantly shortened,the fresh weight and dry weight decreased,and the photosynthetic rate,transpiration rate,stomatal conductance and photochemical efficiency of transgenic A. thaliana decreased,and the expression of photosynthesis-related genes in transgenic A. thaliana was also down-regulated. It is suggested that AsHSP26.8a inhibits the expression of photosynthesis-related genes in transgenic A. thaliana,which weakened the photosynthesis of transgenic A. thaliana,and subsequently leaded to the inhibition of root growth and dry matter accumulation of transgenic A. thaliana.

Key words: Agrostis stolonifera, chloroplast small heat shock protein, AsHSP26.8a, qRT-PCR, photosynthesis, transgenic, Arabidopsis thaliana

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