Identification of Tomato spotted wilt orthotospovirus in Chifeng,Inner Mongolia

doi:10.16420/j.issn.0513-353x.2020-0750

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (6): 1208-1216.doi: 10.16420/j.issn.0513-353x.2020-0750

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Identification of Tomato spotted wilt orthotospovirus in Chifeng,Inner Mongolia

YANG Fei¹, ZHANG Aihong¹, WANG Xiuzhi², LI Xiwang¹, DI Dianping¹^,^*, MIAO Hongqin¹, ZHANG Shangqing³, SUN Xiangrui¹, SUN Qian¹

¹Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture,Baoding,Hebei 071000,China
²Chifeng Academy of Agricultural and Animal Sciences,Chifeng,Inner Mongolia 024031,China
³Tangshan Academy of Agricultural Sciences,Tangshan,Hebei 063001,China

Received:2020-11-18 Revised:2021-03-10 Online:2021-06-25 Published:2021-07-08
Contact: DI Dianping

Abstract

Abstract:

In this study,both tomato spotted wilt orthotospovirus（TSWV）universal primers and TSWV synthetic primers were used to detect the virus disease samples collected from parts of Chifeng,Inner Mongolia China,and the amplified sequences were analyzed. Then,the nucleotide sequence containing N gene amplified by using specific primers was sequenced for phylogenetic analysis. The results showed that TSWV was detected in all 18 tomato fruit samples. The phylogenetic analysis of sequences including 914 nt fragment containingN gene showed that TSWV tomato isolate from Inner Mongolia was more closely to isolates from Yunnan and Jiangsu. After sap inoculation,pepper showed the symptoms of chlorosis in leaf and dwarfing. TSWV was detected by RT-PCR on the symptomatic leaves of pepper. This is the first time to prove that TSWV is harmful to tomato by molecular methods in Inner Mongolia.

Key words: tomato, tomato spotted wilt orthotospovirus, RT-PCR, Inner Mongolia

CLC Number:

S641.2

YANG Fei, ZHANG Aihong, WANG Xiuzhi, LI Xiwang, DI Dianping, MIAO Hongqin, ZHANG Shangqing, SUN Xiangrui, SUN Qian. Identification of Tomato spotted wilt orthotospovirus in Chifeng,Inner Mongolia[J]. Acta Horticulturae Sinica, 2021, 48(6): 1208-1216.

Figures/Tables 6

References 29

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序号 No.	Genbank登录号 Genbank Accession No.	地区 Area	寄主 Host
1	MN365037	中国云南Yunnan China	花生Arachis hypogaea
2	MF688996	中国云南Yunnan China	桔梗Platycodon grandiflorus
3	KY495612	中国云南Yunnan China	刺天茄Solanum indicum
4	KU976396	中国江苏Jiangsu China	莴苣Lactuca sativa
5	MG878875	中国黑龙江Helongjiang China	番茄Solanum lycopersicum
6	MF422034	中国云南Yunnan China	旱金莲Tropaeolum majus
7	KU356854	中国云南Yunnan China	芹菜Apium graveliens
8	MG593199	美国USA	菊苣Cichorium intybus
9	KU179603	美国USA	球兰Hoya carnosa
10	KC261949	韩国Korea	辣椒Capsicum annuum
11	KU179561	美国USA	番茄Solanum lycopersicum
12	KU179533	美国USA	辣椒Capsicum annuum
13	MG983521	意大利Italy	辣椒Capsicum annuum
14	KC261958	韩国Korea	辣椒Capsicum annuum
15	KC261961	韩国Korea	山莴苣Lactuca indica
16	KC261970	韩国Korea	番茄Solanum lycopersicum
17	KM365066	澳大利亚Australia	番茄Solanum lycopersicum
18	AB088385	日本Janpan	—
19	MG602673	南非South Africa	菊花Dendranthema morifolium
20	AJ418781	德国Germany	金钱草Lysimachia christinaeHance
21	KJ649612	匈牙利Hungary	辣椒Capsicum annuum
22	MH367502	土耳其Turkey	番茄Solanum lycopersicum

序号 No.	Genbank登录号 Genbank Accession No.	地区 Area	寄主 Host
1	MN365037	中国云南Yunnan China	花生Arachis hypogaea
2	MF688996	中国云南Yunnan China	桔梗Platycodon grandiflorus
3	KY495612	中国云南Yunnan China	刺天茄Solanum indicum
4	KU976396	中国江苏Jiangsu China	莴苣Lactuca sativa
5	MG878875	中国黑龙江Helongjiang China	番茄Solanum lycopersicum
6	MF422034	中国云南Yunnan China	旱金莲Tropaeolum majus
7	KU356854	中国云南Yunnan China	芹菜Apium graveliens
8	MG593199	美国USA	菊苣Cichorium intybus
9	KU179603	美国USA	球兰Hoya carnosa
10	KC261949	韩国Korea	辣椒Capsicum annuum
11	KU179561	美国USA	番茄Solanum lycopersicum
12	KU179533	美国USA	辣椒Capsicum annuum
13	MG983521	意大利Italy	辣椒Capsicum annuum
14	KC261958	韩国Korea	辣椒Capsicum annuum
15	KC261961	韩国Korea	山莴苣Lactuca indica
16	KC261970	韩国Korea	番茄Solanum lycopersicum
17	KM365066	澳大利亚Australia	番茄Solanum lycopersicum
18	AB088385	日本Janpan	—
19	MG602673	南非South Africa	菊花Dendranthema morifolium
20	AJ418781	德国Germany	金钱草Lysimachia christinaeHance
21	KJ649612	匈牙利Hungary	辣椒Capsicum annuum
22	MH367502	土耳其Turkey	番茄Solanum lycopersicum

Identification of Tomato spotted wilt orthotospovirus in Chifeng,Inner Mongolia

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