Cloning and Prokaryotic Expression of Apple AFS Gene

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (1): 122-124.

• 研究简报 • Previous Articles Next Articles

Cloning and Prokaryotic Expression of Apple AFS Gene

(¹Shandong Academy of Agricultural Sciences, Ji'nan 250100, China; ²College of Life Sciences, Shandong AgriculturalUniversity, Tai'an 271018, China)

Received:2005-01-17 Revised:2005-04-19 Online:2006-02-25 Published:2006-02-25

Abstract

Abstract: α-farnesene synthase (AFS) gene from apple peel tissue was amplified by RT-PCR, and cloned into pET-30a ( + ) vector to construct recombinant prokaryotic exp ression vector pET-AFS. After transformation of E. coli and induced by 1 mmol/L isopropyl-β-D-thiogalactopyranoside ( IPTG) , recombinant protein with 66 kD was expressed in pET-AFS system and separated by SDS-PAGE electrophoresis. The maximum of protein was expressed when induced with IPTG for 6 h. The recombinant protein exp ressed as inclusion bodies.

Key words: Apple, α-farnesene, Prokaryotic expression, SDS-PAGE

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Cloning and Prokaryotic Expression of Apple AFS Gene

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