Cloning and Sequencing of PGIP Gene from Prunus mume Sieb

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (1): 125-127.

• 研究简报 • Previous Articles Next Articles

Cloning and Sequencing of PGIP Gene from Prunus mume Sieb

Li Guangping¹; Fang Jinggui¹; Cai Binhua¹; Zhang Zhen¹;Zhang Changqing²

(¹College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; ²Department of Horticulture, Jinling Institute of Technology, Nanjing 210038, China)

Received:2004-12-08 Revised:2005-05-12 Online:2006-02-25 Published:2006-02-25

Abstract

Abstract: A 1 192 bp sequence of polygalacturonase-inhibiting p rotein ( PGIP) gene(Genbank locus:AY764131) was isolated by polymerase chain reaction ( PCR) from Prunus m um e. This sequence had a intron and a full open reading frame encoding the polygalacturonase-inhibiting protein. Its gene and derived protein were both highly homologous to those from Prunus persica and Prunus mahaleb. A conserved leucinerich fragment had existed in the derived protein sequence.

Key words: Prunus mume, PGIP gene, Cloning, Sequencing

Li Guangping;Fang Jinggui;Cai Binhua;Zhang Zhen;Zhang Changqing. Cloning and Sequencing of PGIP Gene from Prunus mume Sieb[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 125-127.

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Cloning and Sequencing of PGIP Gene from Prunus mume Sieb

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