Establishment of an Efficient RAPD Protocol in Pepper and Its Application in Genetic Purity Testing of F1 Seeds

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (1): 77-79.

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Establishment of an Efficient RAPD Protocol in Pepper and Its Application in Genetic Purity Testing of F₁ Seeds

Huang Sanwen, Zhang Baoxi, Guo Jiazhen, Yang Guimei, Zhu Dewei, DuMeizhen, and Yang Jie

(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences , Beijing 100081)

Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

Abstract

Abstract: In this report, a simple and efficient protocol for RAPD assay in pepper was established through optimization in three main procedures DNA extraction, PCR, electrophoresis. The protocol was employed in screening RAPD markers for genetic purity testing of‘Zhongjiao’serial hybrid cultivars. A total of 12 stable and strong RAPDs were identified to distin guish all 9 F₁ s from their parental lines.

Key words: Pepper (Capsicum annuum L. ), RAPD􀀁, PCR, Hybrid purity determination

CLC Number:

S 641.3􀀁

Huang Sanwen;Zhang Baoxi;Guo Jiazhen;Yang Guimei;Zhu Dewei;DuMeizhen;and Yang Jie. Establishment of an Efficient RAPD Protocol in Pepper and Its Application in Genetic Purity Testing of F₁ Seeds[J]. ACTA HORTICULTURAE SINICA, 2001, 28(1): 77-79.

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Establishment of an Efficient RAPD Protocol in Pepper and Its Application in Genetic Purity Testing of F₁ Seeds

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