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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 347-354.doi: 10.16420/j.issn.0513-353x.2020-0241

• Research Notes • Previous Articles     Next Articles

Detection and Sequence Analyses of Grapevine Leafroll-associated Virus 7 Isolates in China

FAN Xudong, DONG Yafeng*(), ZHANG Zunping, REN Fang, HU Guojun, ZHANG Mengyan, LI Chen   

  1. National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
  • Received:2020-09-18 Revised:2020-12-11 Online:2021-02-25 Published:2021-03-09
  • Contact: DONG Yafeng E-mail:yfdong@163.com

Abstract:

Grapevine leafroll disease(GLD)is a virus disease widely distributed in the world and causes great damage on grapevine. Grapevine leafroll-associated virus 7(GLRaV-7) is one of its pathogens. To investigate the genetic variation of GLRaV-7 isolates,42 positive samples were screened out by reverse transcription-PCR(RT-PCR)from 188 grapevine samples which were collected from 15 Chinese provinces and regions. The detection rate of GLRaV-7 was 24.5%. The coat protein(CP)genes of 42 isolates and heat-shock protein 70(HSP70)genes of 15 isolates were sequenced and analyzed respectively,and the results showed identities ranging 89.12%-100.00% and 88.94%-100.00%,85.33%-100.00% and 88.97%-100.00% at the nucleotide and amino acid sequences respectively. Phylogenetic analysis of CP and HSP70 gene sequences showed that all of GLRaV-7 isolates can be divided into six and five groups,respectively. Gp3,Gp4 and Gp5 only included GLRaV-7 Chinese isolates obtained in this study. This is the first report on the genetic variation of CP and HSP70 genes of GLRaV-7 isolates in China,and this study will be helpful for pathogenicity studies and development of reliable diagnostic methods.

Key words: grapevine, grapevine leafroll-associated virus 7, RT-PCR detection, CP gene, HSP70 gene, phylogenetic analysis

CLC Number: