Transcriptome Analysis of Flower Buds of Sweet Cherry Cultivars with Different Chilling Requirements During Dormancy Stages in Hangzhou

doi:10.16420/j.issn.0513-353x.2022-0297

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (6): 1187-1202.doi: 10.16420/j.issn.0513-353x.2022-0297

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Transcriptome Analysis of Flower Buds of Sweet Cherry Cultivars with Different Chilling Requirements During Dormancy Stages in Hangzhou

RUAN Ruoxin¹, LUO Huifeng¹, ZHANG Chen¹, HUANG Kangkang¹, XI Dujun¹, PEI Jiabo¹, XING Mengyun¹^,², LIU Hui¹^,^*()

¹Institute of Horticulture，Hangzhou Academy of Agricultural Sciences，Hangzhou 310024，China
²Department of Horticulture，Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology，Zhejiang University，Hangzhou 310058，China

Received:2023-01-28 Revised:2023-05-12 Online:2023-06-25 Published:2023-06-27
Contact: * （E-mail：liuhui518lh@163.com）

Abstract

Abstract:

This study aims to explore the molecular regulation mechanism of flower bud dormancy in sweet cherry cultivars that are suitable for growing in South China. Two sweet cherry cultivars（‘Brooks’and‘Summit’）planted in Hangzhou with different chilling requirements were used as materials. Flower buds were collected at three different dormancy stages，namely pre-dormancy stage（S1），endodormancy stage（S2），and pre-budbreak stage（S3）. Transcriptome sequencing was performed to analyze the gene expression differences between these two cultivars. 343，671，and 1 588 differentially expressed genes （DEGs）were obtained in S1，S2，and S3，respectively，and the number of DEGs in S3 was the largest. GO analysis revealed that the DEGs between the two cultivars were mainly enriched in the processes related to cellular component organization，cellular metabolism，response to stimulus，and transporter activity in the dormancy stages. KEGG analysis showed that the DEGs concentrated in S3，which involved sugar metabolism，protein processing，plant-pathogen interaction，and plant hormone signal transduction. The expression trends of some DEGs suggested their roles in regulating the transition of dormancy state of flower buds in sweet cherry. Forty-two differentially expressed transcription factors including nine families were obtained by predictive analysis of transcription factors. The expression levels of 13 transcription factors belonging to AP2/ERF，MYB，WRKY，C2H2，C3H，and MADS-box family in S2 were significantly higher than those in S1 and S3，suggesting that these transcription factors may be involved in the regulation of flower bud dormancy.

Key words: sweet cherry, flower bud, dormancy, transcriptome, DEGs

CLC Number:

S662.5

RUAN Ruoxin, LUO Huifeng, ZHANG Chen, HUANG Kangkang, XI Dujun, PEI Jiabo, XING Mengyun, LIU Hui. Transcriptome Analysis of Flower Buds of Sweet Cherry Cultivars with Different Chilling Requirements During Dormancy Stages in Hangzhou[J]. Acta Horticulturae Sinica, 2023, 50(6): 1187-1202.

Figures/Tables 8

Fig. 1 The analysis of effective chilling accumulation during dormancy stages of sweet cherry at sampling site

Fig. 2 Number（A）and Venn diagram（B）of DEGs in flower buds of sweet cherry‘Brooks’and‘Summit’at different dormancy stages S1：Pre-dormancy stage；S2：Endormancy stage；S3：Pre-budbreak stage. The same below.

Fig. 3 Principal component analysis (PCA) of DEGs in flower buds of sweet cherry ‘Brooks’ and ‘Summit’ at different dormancy stages

Fig. 4 GO enrichment analysis of DEGs in flower buds of sweet cherrry‘Brooks’and‘Summit’at different dormancy stages

Fig. 5 KEGG enrichment of DEGs in flower buds of sweet cherry ‘Brooks’ and ‘Summit’ at different dormancy stages

Fig. 6 Expression profiles of DEGs involved in carbohydrate metabolism related pathways between flower buds of sweet cherry‘Brooks’and‘Summit’at different dormancy stages The values shown in the heatmap represent the average relative gene expression levels（log2FC）of DEGs in‘Summit’versus‘Brooks’at the same stage.

Fig. 7 Expression profiles of DEGs involved in stress response-related pathways between flower buds of sweet cherry‘Brooks’and‘Summit’at different dormancy stages The values shown in the heatmap represent the average relative gene expression levels（log2FC）of DEGs in‘Summit’versus‘Brooks’at the same stage.

Fig. 8 Expression profile of DEGs encoded trancription factors in flower buds of sweet cherry‘Brooks’and‘Summit’at different dormancy stages

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Transcriptome Analysis of Flower Buds of Sweet Cherry Cultivars with Different Chilling Requirements During Dormancy Stages in Hangzhou

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