Characterization of NCL Family Genes in Malus and Their Relationship with Cellular Calcium Concentration in Root

doi:10.16420/j.issn.0513-353x.2021-1267

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 475-484.doi: 10.16420/j.issn.0513-353x.2021-1267

• Research Papers • Previous Articles Next Articles

Characterization of NCL Family Genes in Malus and Their Relationship with Cellular Calcium Concentration in Root

NING Yuansheng, LI Huan, SONG Jianfei, YU Tingting, HAN Mengyuan, PENG Lulin, JIA Junqi, ZHANG Weiwei, YANG Hongqiang^*()

State Key Laboratory of Crop Biology，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China

Received:2022-06-17 Revised:2022-11-09 Online:2023-03-25 Published:2023-04-03
Contact: *（E-mail：hqyang@sdau.edu.cn）

Abstract

Abstract:

Na⁺/Ca²⁺exchange-like proteins（NCX-Like，NCL）take part in the transmembrane transport of Ca²⁺ and other cations in Arabidopsis. The traits of NCL family gene and expression patterns of apple MdNCL based on Arabidopsis NCL sequences and genome-wide information of apple，grape，strawberry，peach and pear were analyzed；and the relationship between MhNCL expression and cellular Ca²⁺ concentration in the roots of Malus hupehensis Rhed. under different treatments were investigated. Results showed that the NCL family members were highly conserved in evolution；four MdNCLs originated from apple genome all contained cis-acting components of abiotic stress response and hormone response on their promoters and had organ-specific expression patterns. Based on the conserved structural domains of MdNCL，four MhNCLs（MhNCL-1，MhNCL-2，MhNCL-3 and MhNCL-4）were identified in M. hupehensis. Expression levels of MhNCL-1，MhNCL-3 and MhNCL-4 showed a“little change-increase-decrease-increase”pattern under NaCl and CaCl₂treatments，and showed a“little change-increase-decrease-increase”pattern under ABA and IAA treatments. The Ca²⁺concentration in root cells showed an“increase-decrease-small increase”trend under NaCl and CaCl₂treatments，and an“increase-decrease-increase-decrease”pattern under ABA and IAA treatments，which indicated that there was a correlation between the expression levels of MhNCLs and changes in cellular Ca²⁺concentration in root.

Key words: apple, NCL gene, genome-wide identification, Ca²⁺concentration

CLC Number:

S661.1

NING Yuansheng, LI Huan, SONG Jianfei, YU Tingting, HAN Mengyuan, PENG Lulin, JIA Junqi, ZHANG Weiwei, YANG Hongqiang. Characterization of NCL Family Genes in Malus and Their Relationship with Cellular Calcium Concentration in Root[J]. Acta Horticulturae Sinica, 2023, 50(3): 475-484.

Figures/Tables 7

References 28

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基因名称 Gene name	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Revers primer sequence
MhNCL-1	GGTGAACCTGATGCAGACAT	TCAGGCTTACTGATACGGGA
MhNCL-2	GTCTAACAACTCCACTGTGC	GCAATAACTCGCTCCCATTC
MhNCL-3	AGCTTCTCAACAGCTACGAG	ACCAGACCCAAGAATACTGC
MhNCL-4	CAAGTTTGGTAAGATTCCGGG	CTGTTTCGTCCCATGTCCTT
MdActin	ATTCAAGTATGCCTGGGTGC	CAGTCAGCCTGTGATGTTCC

基因名称 Gene name	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Revers primer sequence
MhNCL-1	GGTGAACCTGATGCAGACAT	TCAGGCTTACTGATACGGGA
MhNCL-2	GTCTAACAACTCCACTGTGC	GCAATAACTCGCTCCCATTC
MhNCL-3	AGCTTCTCAACAGCTACGAG	ACCAGACCCAAGAATACTGC
MhNCL-4	CAAGTTTGGTAAGATTCCGGG	CTGTTTCGTCCCATGTCCTT
MdActin	ATTCAAGTATGCCTGGGTGC	CAGTCAGCCTGTGATGTTCC

物种 Species	基因名称 Gene name	基因登录号 Gene ID	氨基酸 aa	等电点 pI	分子量/kD Molecular weight	染色体 Chromosome	亚细胞定位 Subcellular localization
苹果	MdNCL-1	MD03G1238600	586	5.39	63.75	Chr3	细胞膜 Cell membrane
Malus × domestica	MdNCL-2	MD03G1238700	600	5.30	66.03	Chr3	细胞膜 Cell membrane
	MdNCL-3	MD11G1258400	584	5.01	63.26	Chr11	细胞膜 Cell membrane
	MdNCL-4	MD17G1233800	585	5.34	64.56	Chr17	细胞膜 Cell membrane
拟南芥 Arabidopsis thaliana	AtNCL	AT1G53210.1	585	5.15	63.42	Chr1	细胞膜 Cell membrane
葡萄	VvNCL-1	VIT_212s0028g02570	577	5.73	62.98	Chr12	细胞膜 Cell membrane
Vitis vinifera	VvNCL-2	VIT_219s0014g01740	579	5.21	63.10	Chr19	细胞膜 Cell membrane
草莓	FvNCL-1	FvH4_3g18760	578	5.75	63.67	fvb3	细胞膜 Cell membrane
Fragaria vesca	FvNCL-2	FvH4_3g18770	580	5.41	63.51	fvb3	细胞膜 Cell membrane
	FvNCL-3	FvH4_6g26990	580	4.81	63.58	fvb3	细胞膜 Cell membrane
桃	PpNCL-1	Prupe.3G125200	575	5.56	63.45	pp03	细胞膜 Cell membrane
Prunus persica	PpNCL-2	Prupe.4G171300	593	5.39	64.77	pp04	细胞膜 Cell membrane
	PpNCL-3	Prupe.4G171400	579	5.30	63.26	pp04	细胞膜 Cell membrane
白梨	PbNCL-1	Pbr038243.1	585	5.05	63.61	Chr11	细胞膜 Cell membrane
Pyrus bretschneideri	PbNCL-2	Pbr022502.1	585	5.23	64.46	Chr17	细胞膜 Cell membrane
	PbNCL-3	Pbr002291.1	549	5.26	60.01	scaffold1094.0	细胞膜 Cell membrane
	PbNCL-4	Pbr002292.1	580	5.18	63.69	scaffold1094.0	细胞膜 Cell membrane
	PbNCL-5	Pbr002293.1	580	5.18	63.69	scaffold1094.0	细胞膜 Cell membrane
	PbNCL-6	Pbr002294.1	549	5.26	60.01	scaffold1094.0	细胞膜 Cell membrane

物种 Species	基因名称 Gene name	基因登录号 Gene ID	氨基酸 aa	等电点 pI	分子量/kD Molecular weight	染色体 Chromosome	亚细胞定位 Subcellular localization
苹果	MdNCL-1	MD03G1238600	586	5.39	63.75	Chr3	细胞膜 Cell membrane
Malus × domestica	MdNCL-2	MD03G1238700	600	5.30	66.03	Chr3	细胞膜 Cell membrane
	MdNCL-3	MD11G1258400	584	5.01	63.26	Chr11	细胞膜 Cell membrane
	MdNCL-4	MD17G1233800	585	5.34	64.56	Chr17	细胞膜 Cell membrane
拟南芥 Arabidopsis thaliana	AtNCL	AT1G53210.1	585	5.15	63.42	Chr1	细胞膜 Cell membrane
葡萄	VvNCL-1	VIT_212s0028g02570	577	5.73	62.98	Chr12	细胞膜 Cell membrane
Vitis vinifera	VvNCL-2	VIT_219s0014g01740	579	5.21	63.10	Chr19	细胞膜 Cell membrane
草莓	FvNCL-1	FvH4_3g18760	578	5.75	63.67	fvb3	细胞膜 Cell membrane
Fragaria vesca	FvNCL-2	FvH4_3g18770	580	5.41	63.51	fvb3	细胞膜 Cell membrane
	FvNCL-3	FvH4_6g26990	580	4.81	63.58	fvb3	细胞膜 Cell membrane
桃	PpNCL-1	Prupe.3G125200	575	5.56	63.45	pp03	细胞膜 Cell membrane
Prunus persica	PpNCL-2	Prupe.4G171300	593	5.39	64.77	pp04	细胞膜 Cell membrane
	PpNCL-3	Prupe.4G171400	579	5.30	63.26	pp04	细胞膜 Cell membrane
白梨	PbNCL-1	Pbr038243.1	585	5.05	63.61	Chr11	细胞膜 Cell membrane
Pyrus bretschneideri	PbNCL-2	Pbr022502.1	585	5.23	64.46	Chr17	细胞膜 Cell membrane
	PbNCL-3	Pbr002291.1	549	5.26	60.01	scaffold1094.0	细胞膜 Cell membrane
	PbNCL-4	Pbr002292.1	580	5.18	63.69	scaffold1094.0	细胞膜 Cell membrane
	PbNCL-5	Pbr002293.1	580	5.18	63.69	scaffold1094.0	细胞膜 Cell membrane
	PbNCL-6	Pbr002294.1	549	5.26	60.01	scaffold1094.0	细胞膜 Cell membrane

Characterization of NCL Family Genes in Malus and Their Relationship with Cellular Calcium Concentration in Root

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