https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 487-504.doi: 10.16420/j.issn.0513-353x.2020-0438

• Research Papers • Previous Articles     Next Articles

Dynamic Changes of Auxin and Analysis of Differentially Expressed Genes in Walnut Endocarp During Hardening

YU Shangqi1,2,4, ZHANG Rui2,3,4,*(), GUO Zhongzhong1,2, SONG Yan2,3, FU Jiazhi2,3, WU Pengyu2,3, MA Zhihao3   

  1. 1College of Life Science,Tarim University,Alar,Xinjiang 843300,China
    2Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Alar,Xinjiang 843300,China
    3College of Plant Science and Technology,Tarim University,Alar,Xinjiang 843300,China
    4The National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang,Alar,Xinjiang 843300,China
  • Received:2020-11-16 Online:2021-03-25 Published:2021-04-02
  • Contact: ZHANG Rui E-mail:zhrgsh@163.com

Abstract:

In order to study the hardening of the walnut endocarp,which mainly involves the biological process and the role of auxin in the hardening process,the‘Xinlu’walnut was used as the research material. There is a close relationship between walnut endocarp hardening and distribution of vascular bundles on the walnut endocarp surface by morphological observation and lignin deposition analysis;The hardening of walnut endocarp near the vascular tissue is earlier than that away from the vascular tissue. The content of IAA showed a V-shaped change in the hardening of the endocarp. The average content of IAA was the highest than others during the five stages,16.467 ng·g-1,which was about 6.8 times of the sum of IBA,ICA and ME-IAA. The analysis of intermediate metabolites of lignin synthesis showed that the content of p-coumaryl alcohol of lignin monomer in the hardening area of the endocarp was 16 times of the unhardening area. The content of sinapyl alcohol in the hardening area of the walnut endocarp was extremely high,but it was not detected in the unhardening area,and the content of these two substances in the hardening area of the walnut endocarp showed an overall upward trend with the hardening process. Through transcriptome and proteome correlation analysis,369 differentially expressed genes related to endocarp hardening were obtained. The correlation result of genes that are differentially expressed at both the transcription and protein levels is good,with the lowest correlation coefficient of 0.48124,but the correlation result of all differentially expressed genes is poor,with the highest correlation coefficient of 0.22112. Using Metascape to construct a network of relationships among the top 20 biological functions that are significantly enriched by differentially expressed genes,it is found that four biological processes,namely Phenylpropanoid metabolic process,Response to oxidative stress,Cell wall organization or biogenesis,Amino sugar and nucleotide sugar metabolism were mainly involved in endocarp hardening mainly involve. The fluorescence quantitative results showed that the expression changes trend of AUX/IAA genes JrIAA9,JrIAA16 and JrIAA27 were basically consistent with that of IAA content. It is speculated that IAA plays an important regulatory role in the process of walnut endocarp hardening,and it is of great significance to carry out the research on the mechanism of Bared-nut later.

Key words: walnut, auxin, endocarp, transcriptome, proteome, correlation analysis, lignin

CLC Number: