Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (10): 1907-1920.doi: 10.16420/j.issn.0513-353x.2021-0406

• Research Papers • Previous Articles     Next Articles

Phenotype Classification Based on Flower Color,Pigment Distribution and Epidermal Cell Shape of Dendrobium Hybrids

YIN Hantai1,3,4, YIN Junmei2,4,*(), LIAO Yi3,4, LU Shunjiao3,4, LI Chonghui3,4,*()   

  1. 1College of Horticulture,Hainan University,Haikou 570228,China
    2Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China
    3Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture,Haikou 571101,China
    4The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization,Danzhou,Hainan 571737,China
  • Received:2021-07-19 Revised:2021-09-02 Online:2021-10-25 Published:2021-11-01
  • Contact: YIN Junmei,LI Chonghui;


The color of petal and labellum of 107 Dendrobium hybrid cultivars were measured with a spectrophotometer for cluster analysis. The anatomical structure of petal and labellum obtained by tissue sections was observed to investigate the pigment distribution pattern, the difference of epidermal cell shape and their correlation with flower color phenotype. The results showed that,all cultivars were clustered to eight groups based on the cluster analysis of color description by the CIELab color system. The groups were white,pink,yellow green,purple red,light purple,purple,deep purple,and multicolor. The flower color of Dendrobium hybrids are not diverse,and mainly belong to purple tones. The color of labellum was darker and richer than that of petal. Observation of petals of 72 representative species showed that there were 13 anthocyanin distribution patterns. The wider the anthocyanin distribution was,the lightness of the flower color would decrease and the color would darken. Interestingly,the anthocyanoplasts(ACP)which can enhance the color intensity had been found in one cultivar. Carotenoids were widely distributed in various tissues of petal. There were five types of epidermal cells in the flowers(flat cells,domed epidermal cells,elongated dome cells,papillate cells,epidermal hair cells). Usually,the upper epidermal cells were more conical than lower epidermis. The degree of cone in upper epidermal cells was greater in labellum than that in petal. The a* value and the chroma C value were positively correlated with the height-to-width ratio of petal epidermal cells in cultivars rich in anthocyanins. The a* value and the L* value were positively correlated while the chroma C value was negatively correlated with the height-to-width ratio of petal epidermal cells in cultivars without anthocyanins. Furthermore, the conicity of flower epidermal cells containing carotenoid was significantly less than that of the group without carotenoids.

Key words: Dendrobium, flower color, phenotype classification, flower pigment distribution, epidermal cell shape, anthocyanin, carotenoid, cluster analysis

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