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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1957-1966.doi: 10.16420/j.issn.0513-353x.2021-0630

• Research Papers • Previous Articles     Next Articles

Analysis the Function of R2R3 MYB Transcription Factor RhMYB113c on Regulating Anthocyanin Synthesis in Rosa hybrida

LI Maofu1,2,3, YANG Yuan1,2,4, WANG Hua1,2,3, FAN Youwei1,2,3, SUN Pei1,2,3, JIN Wanmei1,2,3,*()   

  1. 1Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China
    2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture and Rural Affairs,Beijing 100093,China
    3Beijing Engineering Research Center of Functional Floriculture,Beijing 100093,China
    4Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China
  • Received:2021-07-02 Revised:2021-09-10 Online:2022-09-25 Published:2022-10-08
  • Contact: JIN Wanmei E-mail:jwm0809@163.com

Abstract:

An R2R3 MYB gene,named RhMYB113c,was cloned from cDNA of the petal in Rosa hybrida‘Carola’. The open reading frame of RhMYB113c was 699 base pair,encoding 233 amino acids. The multiple sequence alignment analysis showed that RhMYB113c contained R2 and R3 domains. Phylogenetic tree results indicated that RhMYB113c was closely related to anthocyanin synthesis activator AtMYB114,AtMYB113,AtMYB75,and AtMYB90. Tissue specific expression showed that RhMYB113c was mainly expressed in petals and styles in‘Carola’,but not in all tissues of white flower variety ‘White Satin’. Gene expression at different flower development stages indicated that RhMYB113c was not expressed in‘Carola’uncolored petals,highly expressed in full flowering stage,but not in all stage of white flower varieties‘White Satin’. Yeast two hybrid and tobacco transient expression experiments suggested that RhMYB113c may regulate anthocyanin synthesis and determine the color of rose petals.

Key words: Rosa, petal, RhMYB113c, anthocyanin synthesis

CLC Number: