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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 166-174.doi: 10.16420/j.issn.0513-353x.2020-0243

• Research Notes • Previous Articles     Next Articles

Optimization of Callus Induction from Immature Embryo and Establishment Regeneration System of Paeonia ostii‘Fengdan’

LIU Rong, CI Huiting, REN Xiuxia, GAO Jie, WANG Shunli(), ZHANG Xiuxin()   

  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,P. R. China,Research Center of Peony,Chinese Academy of Agricultural Sciences,National Center for Flower Improvement,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2021-10-20 Revised:2021-11-18 Online:2022-01-25 Published:2022-01-24

Abstract:

In order to improve callus induction quality,and establish highly efficient regeneration system of Paeonia ostii‘Fengdan’,the immature embryo collecting time,technical treatments of immature embryos,basic medium selection,and different concentrations and combinations of plant growth regulators and H2O2 were optimized. The results showed that the best collection time for immature embryos callus induction was 60 days after pollination. Compared with four technical treatments of immature embryos,thoroughly divided embryos(slices)were produced better callus with low callus browning rate than that with whole embryos,halved embryos,and broken into quarters embryos. MS basic medium with 0.162 μmol · L-1 H2O2 can improve the callus induction rate of immature embryo,and it can also increases the callus browning rate. Different concentration of H2O2 adding in WPM basic medium could not be improved the callus induction rate. WPM basic medium is better than MS basic medium for immature embryo callus induction. The combination of WPM + 2.5 mg · L-1 2,4-D + 0.1 mg · L-1 6-BA can obtain higher callus induction rate(93.33% ± 3.53%)with lower callus browning rate(25.33% ± 4.81%). The best hormone combinations for adventitious bud and root induction were WPM + 0.5 mg · L-1 GA3 + 0.5 mg · L-1 6-BA,1/2 WPM + 2.0 mg · L-1 IBA + 1.0 mg · L-1 IAA,respectively.

Key words: tree peony, immature embryo, callus induction, callus browning, in vitro, regeneration

CLC Number: