Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 233-242.doi: 10.16420/j.issn.0513-353x.2020-0350

• Research Papers • Previous Articles     Next Articles

Identification of TIFY Family in Apple and Their Expression Analysis Under Insect Stress

MEI Chuang1, ZHANG Xiaoyan2, YAN Peng1,*(), Aisajan Mamat1, FENG Beibei1, MA Kai1, HAN Liqun1, DONG Lianxin2, WANG Jixun1,*()   

  1. 1Research Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology(Xinjiang),Ministry of Agriculture Urumqi 830091,China
    2College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi 830052,China
  • Received:2020-11-29 Revised:2021-01-18 Online:2021-02-25 Published:2021-03-09
  • Contact: YAN Peng,WANG Jixun;


The TIFY transcription factors in the apple were identified using bioinformatics methods. The gene structure,conserved motifs and evolutionary relationship were analyzed. Meanwhile,the differential expression of apple TIFY family member genes under insect Agrilus mari stress conditions was determined based on Malus sieversii transcriptome data analysis. A total of 16 TIFY transcription factors were identified through analysis,which encodes proteins between 209-449 aa. The 16 MdTIFY genes are located on the 8 chromosomes of apples,and all MdTIFY proteins have conserved TIFY domains. Among them,eight MdTIFY correspond to the spliced transcripts of M. sieversii transcriptome sequencing. These genes all contain conserved TIFY functional domains. Multi-species phylogeny analyses showed that these proteins can be clustered into 7 groups. The expression levels of the seven TIFY genes showed significant differences among different treatments. After pest infestation,the expression of MsTIFY10B-a increased by 34-fold in insect-resistant plants,and the expression of MsTIFY9-c increased by 5.2-fold. Meanwhile the contents of jasmonic acid-isoleucine(JA-Ile)in insect-resistant plants were significantly higher than that of sensitive plants. The important insect-resistance response genes,MsTIFY10B-a and MsTIFY9-c,are located in the key nodes of association network in M. sieversii,being as important regulators during the plant responses tobiotic stresses.

Key words: Malus sieversii, gene family, TIFY, expression analysis, jasmonate

CLC Number: