Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 739-748.doi: 10.16420/j.issn.0513-353x.2021-0179

• Research Papers • Previous Articles     Next Articles

Expression of E-type MADS-box Genes in Flower and Fruits and Protein Interaction Analysis in Chinese Jujube

GAO Weilin1, ZHANG Liman1, XUE Chaoling1, ZHANG Yao1, LIU Mengjun2, ZHAO Jin1,*()   

  1. 1. College of Life Science,Hebei Agricultural University,Baoding,Hebei 071000,China
    2. College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2021-11-18 Revised:2022-01-18 Online:2022-04-25 Published:2022-04-24
  • Contact: ZHAO Jin


In order to study the function of E-type MADS-box genes in flower and fruit of Chinese jujube(Ziziphus jujuba Mill.),their bioinformatics analysis,expression pattern,subcellular location and protein interaction in‘Jinsi Xiaozao’were studied systematically. A total of three E-type MADS-box genes were identified in the jujube genome,which are located in the three clades of SEP1/2,SEP3 and SEP4. Compared with homologous sequences of other species,they are very conservative in evolution. Three E-type genes expressed in jujube flowers and fruits. The expression of ZjSEP1/2 was relatively stable at different stages of flower development,ZjSEP3 and ZjSEP4 were highly expressed in the early stage of flower development. After phytoplasma infection,their expressions in phyllody,a typical diseased symptom, were inhibited dramatically. All three proteins were located in the nucleus. According to the results of yeast one-hybrid analysis,ZjSEP1/2 and ZjSEP3 proteins had transcriptional activation activity and could be used as transcription factors to regulate downstream genes,while ZjSEP4 didn’t have this activity. According to the yeast two-hybrid results,all three proteins could interact with A-type and C/D-type proteins. Both ZjSEP4 and ZjSEP1/2 could interact with itself,but ZjSEP3 couldn’t interact with itself. In summary,the three E-type genes play an important role in the development of jujube flowers,but they should have various regulatory modes. ZjSEP3 couldn’t form homodimers,while ZjSEP4 and ZjSEP1/2 could form homodimers. But all of three proteins could form heterodimers with A-type and C/D-type proteins.

Key words: Ziziphus jujuba, MADS-box gene, E-type, flower and fruit development, expression analysis, transcriptional activation, protein interaction

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