Analysis of Proliferation Abilities and Telomere Lengths of Apple Plantlets in Vitro Preserved for Eight to Thirty-three Years

doi:10.16420/j.issn.0513-353x.2020-0305

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 243-253.doi: 10.16420/j.issn.0513-353x.2020-0305

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Analysis of Proliferation Abilities and Telomere Lengths of Apple Plantlets in Vitro Preserved for Eight to Thirty-three Years

LIU Yajie^*, LIANG Chen^*, WANG Li, DU Guoqiang^**(), SHI Xiaoxin^**()

College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001,China

Received:2020-09-16 Revised:2020-12-27 Online:2021-02-25 Published:2021-03-09
Contact: DU Guoqiang,SHI Xiaoxin E-mail:gdu@hebau.edu.cn;shixx@hebau.edu.cn

Abstract

Abstract:

In order to examine the relationship between telomere lengths and their propagation ability or aging,and to explore the relationship between the telomer lengths and the preservation time limit in apple plantlets in vitro,the phenotypes,proliferation characteristics,and telomere lengths of apple plantlets preserved in vitro and consecutively subcultured for years were studied in three apple cultivars. The results showed that there were no changes in the proliferation abilities and no significant changes in phenotypes in most apple shoot plantlets in vitro from 8 to 33-year‘Fuji’preserved,8 to 27-year‘Golden Delicious’preserved,and 8 to 25-year‘Gala’preserved,respectively. No significant change in telomere lengths was found in leaves of the plantlets at 30-day plantlet age with different subculture times in three cultivars. The telomere lengths in the 73th subculture time plantlets could change during the given subculture periods, such as 10,30,50,and 90 days,which showed cultivar specificity. For example,there was no significant difference of telomere lengths in plantlet leaves of‘Fuji’and‘Gala’,while the telomere lengths in plantlet leaves of‘Golden Delicious’were the longest at 50-day and the shortest at 90-day. The telomere lengths in the 71th subculture time plantlet stems cultured for 30 days of‘Golden Delicious’,were significantly higher than that in leaves,and no significant difference was found either between those in stems and calli or in leaves and calli;while in‘Gala’,the telomere lengths in calli were the longest,followed by that in leaves and stems. The changes of the telomere lengths in apple plantlets in vitro coincided with their proliferation abilities,phenotypes,and aging during the same subculture time.

Key words: Malus × domestica;, subculture time, plantlet in vitro, plantlet age, callus, telomere length

CLC Number:

S661.1

LIU Yajie, LIANG Chen, WANG Li, DU Guoqiang, SHI Xiaoxin. Analysis of Proliferation Abilities and Telomere Lengths of Apple Plantlets in Vitro Preserved for Eight to Thirty-three Years[J]. Acta Horticulturae Sinica, 2021, 48(2): 243-253.

Figures/Tables 7

References 45

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品种 Cultivar	外植体接种日期/ （Y-M-D） Date of explant inoculated	保存时间/年 Years of preserved time	继代次数 Subculture times	出梢数 Number of shoots	有效新梢数 Number of effective shoots
‘富士’ ‘Fuji’	1985-04-20	33	229	5.75 ± 1.33 a	2.15 ± 0.50 a
	1996-05-23	22	139	5.40 ± 0.59 ab	1.55 ± 0.55 ab
	2005-05-11	13	68	4.85 ± 0.93 ab	1.00 ± 0.75 b
	2007-03-12	11	52	4.70 ± 1.21 ab	1.35 ± 0.34 ab
	2010-03-18	8	31	4.05 ± 0.50 b	1.50 ± 0.53 ab
‘金冠’ ‘Golden Delicious’	1991-04-16	27	180	4.85 ± 0.44 a	2.10 ± 0.26 b
	1996-05-23	22	68	4.95 ± 0.50 a	2.85 ± 0.82 ab
	2005-05-11	13	52	5.00 ± 1.07 a	3.05 ± 0.44 a
	2010-03-18	8	31	4.30 ± 0.26 a	2.70 ± 0.58 ab
‘嘎拉’ ‘Gala’	1993-12-03	25	165	8.50 ± 0.40 a	2.05 ± 0.72 a
	2000-07-26	22	106	8.20 ± 0.72 a	2.05 ± 0.96 a
	2005-05-11	13	68	8.40 ± 0.31 a	1.85 ± 0.34 a
	2007-03-12	11	52	8.30 ± 0.20 a	1.80 ± 0.16 a
	2010-03-18	8	31	8.45 ± 0.92 a	1.70 ± 0.90 a

品种 Cultivar	外植体接种日期/ （Y-M-D） Date of explant inoculated	保存时间/年 Years of preserved time	继代次数 Subculture times	出梢数 Number of shoots	有效新梢数 Number of effective shoots
‘富士’ ‘Fuji’	1985-04-20	33	229	5.75 ± 1.33 a	2.15 ± 0.50 a
	1996-05-23	22	139	5.40 ± 0.59 ab	1.55 ± 0.55 ab
	2005-05-11	13	68	4.85 ± 0.93 ab	1.00 ± 0.75 b
	2007-03-12	11	52	4.70 ± 1.21 ab	1.35 ± 0.34 ab
	2010-03-18	8	31	4.05 ± 0.50 b	1.50 ± 0.53 ab
‘金冠’ ‘Golden Delicious’	1991-04-16	27	180	4.85 ± 0.44 a	2.10 ± 0.26 b
	1996-05-23	22	68	4.95 ± 0.50 a	2.85 ± 0.82 ab
	2005-05-11	13	52	5.00 ± 1.07 a	3.05 ± 0.44 a
	2010-03-18	8	31	4.30 ± 0.26 a	2.70 ± 0.58 ab
‘嘎拉’ ‘Gala’	1993-12-03	25	165	8.50 ± 0.40 a	2.05 ± 0.72 a
	2000-07-26	22	106	8.20 ± 0.72 a	2.05 ± 0.96 a
	2005-05-11	13	68	8.40 ± 0.31 a	1.85 ± 0.34 a
	2007-03-12	11	52	8.30 ± 0.20 a	1.80 ± 0.16 a
	2010-03-18	8	31	8.45 ± 0.92 a	1.70 ± 0.90 a

Analysis of Proliferation Abilities and Telomere Lengths of Apple Plantlets in Vitro Preserved for Eight to Thirty-three Years

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