Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1637-1649.doi: 10.16420/j.issn.0513-353x.2021-0642

• Research Papers • Previous Articles     Next Articles

Genome-wide Identification of Dof Family Genes and Expression Analysis Sepal Persistent and Abscission in Pear

LIU Jinming, GUO Caihua, YUAN Xing, KANG Chao, QUAN Shaowen, NIU Jianxin*()   

  1. Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Department of Horticulture,College of Agriculture,Shihezi University,Shihezi,Xinjiang 832003,China
  • Received:2022-01-07 Revised:2022-05-30 Online:2022-08-25 Published:2022-09-05
  • Contact: NIU Jianxin


Dof genes in pear genome were identified by BLAST software based on Dof genes from domestic apple and Arabidopsis thaliana. TBtools,MEME and MEGA-X software were used for bioinformatics analysis of Dof protein and gene sequences. The results showed that a total of 51 PbDof genes were identified,which belong to nine subfamilies. Structural analysis showed that the number of introns in PbDof gene varies from one to three. The promoter region contains five cis-regulatory elements related to stress response and nine cis-regulatory elements related to plant hormones. Synteny analysis of PbDof genes showed that PbDof was mainly affected by purification selection in the process of species evolution. To provide theoretical basis for understanding what roles the Dof genes play in calyx persistence and abscission of pear,the qRT-PCR method was used to detect the relative expression of Dof genes in ovary and sepals after sprayed with distilled water,PP333 and GA3 at full bloom stage. qRT-PCR analysis revealed that the expression levels of PbDof34 and PbDof51 after GA3 treatment were significantly higher than those after PP333 treatment,and the expression levels of PbDof34 were significantly higher than those after distilled water treatment(control) in calyxes on 9 d. The expression levels of PbDof16,PbDof19 and PbDof44 after GA3 treatment were all lower than those after PP333 treatment in calyxes on 9 d. The expression levels of PbDof16 and PbDof44 after PP333 treatment was significantly lower than those of control in calyxes on 9 d. We speculated that PbDof34 and PbDof51 may promote the calyxes persistence whereas PbDof16,PbDof19 and PbDof44 may promote the calyxes abscission in‘Korla Fragrant Pear’.

Key words: pear, Dof gene family, calyx persistence, calyx abscission

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