Abstract: Seventy-six CML family genes in Brassica oleracea var. capitata were identified and characterized through B.oleracea genomics database and NCBI database.They are randomly distributed on 9 chromosomes. There are 12 genes at the most，and at least 1 gene. It was found that the amino acid residues of the family of encoded proteins ranged from 109 to 365，and the molecular weight was mostly about 20 kD. They were small molecular proteins and contained 2–4 conserved EF-hand domains. Phylogenetic analysis showed that these CML family genes could be classified into eight groups， combining with Arabidopsis CMLs and each subfamily genes have high homology and close evolutionary relationship. Subcellular predictive analysis showed that most of the proteins were expressed in the plasma membrane and extracellular membrane，and a few in the plasma membrane，nucleus and chloroplast. Among the 76 genes，25 genes were differentially expressed after self-pollination and cross-pollination. Among them，8 genes，such as BoCML12，BoCML42，BoCML44，BoCML45，BoCML60，BoCML70，BoCML71and BoCML74，have significant expression differences. The results indicated that these 8 genes were involved in the regulation of self-pollination and cross-pollination of cabbage in different ways and may be related to the self-incompatibility of cabbage.

Key words: Brassica oleracea var. capitata；CML family genes, bioinformatics, expression analysis