Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1505-1518.doi: 10.16420/j.issn.0513-353x.2021-0474

• Research Papers • Previous Articles     Next Articles

Identification and Expression Analysis of MYB Transcription Factors Regulating the Anthocyanin Biosynthesis in Zinnia elegans and Function Research of ZeMYB9

QIAN Jieyu, JIANG Lingli, ZHENG Gang, CHEN Jiahong, LAI Wuhao, XU Menghan, FU Jianxin, ZHANG Chao()   

  1. Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,School of Landscape Architecture,Zhejiang A & F University,Hangzhou 311300,China
  • Received:2022-02-27 Revised:2022-04-24 Online:2022-07-25 Published:2022-07-29


In this study,Zinnia elegans‘Dreamland Red’was employed as plant materials,and 33 MYB transcription factors were screened based on the transcriptome data of Z. elegans. Furthermore,phylogenetic tree analysis revealed that ten R2R3-MYB transcription factors probably related to anthocyanin synthesis were identified in Z. elegans. Among them,five MYB proteins of Z. elegans belonged to subgroup 4(S4),three MYB proteins were clustered in S6,and two MYB proteins were included in S7. According to combined analysis on gene expression levels of ten MYB genes and anthocyanidin content in Z. elegans‘Dreamland Red’,it was suggested that ZeMYB32 of S4 probably negatively regulated anthocyanin synthesis in Z. elegans,while ZeMYB3 and ZeMYB16 promoted the accumulation of anthocyanin. What’s more,ZeMYB9 and ZeMYB10 of S6 probably positively regulated anthocyanin synthesis. Further research found that ZeMYB9 is located in the nucleus. Overexpression of ZeMYB9 gene in tobacco enhanced the marked accumulation of anthocyanins in leaves,and dramatically up-regulated the expression levels of all detected genes related with anthocyanin synthesis,revealing that ZeMYB9 plays a positive role in anthocyanin biosynthesis.

Key words: Zinnia elegans, anthocyanin, MYB transcription factors, bioinformatics analysis

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