Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 655-662.doi: 10.16420/j.issn.0513-353x.2020-1053

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Molecular Detection and Sequence Analysis of ORSV and CymMV in Cultivated Cymbidium goeringii in Dali,Yunnan

CHEN Jianxin1, WEI Yuqian1, TANG Jie1, ZHU Yongjin1, MA Huancheng2, WU Jianrong1,2,**()   

  1. 1Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province,College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China
    2Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration,College of Biodiversity Forestry,Southwest Forestry University,Kunming 650224,China
  • Received:2021-07-07 Revised:2021-11-25 Online:2022-03-25 Published:2022-03-25
  • Contact: WU Jianrong


The survey on virus diseases was carried out and the samples of infected seedlings were collected in Dali,Yunnan,China during 2018—2019. The Reverse Transcription-Polymerase Chain Reaction (RT-PCR)was used to detect the virus. The results showed that total 70 sample were infected by ORSV (odontoglossum ringspot virus). However,CymMV(cymbidium mosaic virus)was not detected,indicating the major potential infection thread may be from ORSV in Dali. Phylogenetic analysis based on Coat Protein gene(cp)showed that all ORSV clustered into a single clade. The results of protein analysis showed that ORSV coat protein belonged to TMV coat protein family,and the theoretical isoelectric point was 5.01,which was a hydrophilic stable protein. Thus,we confirmed that ORSV had infected in cultivated C. goeringii in Dali. The follow up study should be carried out soon on early diagnosing and screening of anti-virus medicine for orchid viral disease.

Key words: Cymbidium goeringii, CymMV, ORSV, molecular detection, cp gene, bioinformatics

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