QTL Mapping and Identification of Candidate Gene for Resistance to Gummy Stem Blight in Cucumis sativus/hystrix Introgression Line‘IL77’

doi:10.16420/j.issn.0513-353x.2018-0269

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (11): 2141-2152.doi: 10.16420/j.issn.0513-353x.2018-0269

• Research Papers • Previous Articles Next Articles

QTL Mapping and Identification of Candidate Gene for Resistance to Gummy Stem Blight in Cucumis sativus/hystrix Introgression Line‘IL77’

ZHANG Xu，XU Jian，LI Ji，LOU Qunfeng，and CHEN Jinfeng*

（College of Horticulture，State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University，Nanjing 210095，China）

Online:2018-11-25 Published:2018-11-25

Abstract

Abstract: In this study，a linkage map containing 137 pairs of SSR markers and 6 pairs of InDel markers was constructed using recombinant inbred lines F2︰6 derived from a cross between parental lines‘IL77’（resistant line from the cross of C. hystrix × C. sativus）and‘8419’（susceptible line）. Genetic analysis indicated that the resistance to gummy stem blight（GSB）in‘IL77’was quantitative. Both mapping and BSA-seq analysis were used to detect quantitative trait loci（QTLs）conferring GSB resistance in cucumber leaves. The results showed that the GSB resistance genes were located in the 24.6–27.1 Mb region of chromosome 1. There were 13 candidate genes with exonic nonsynonymous SNP mutation predicted as GSB-resistance related genes in this region. Moreover，bioinformatics analysis and qRT-PCR results provided strong evidence that Csa1G654870 was closely related to GSB resistance process.

Key words: cucumber, introgression line, gummy stem blight, linkage map, BSA-seq, resistance gene

CLC Number:

S 642.2

ZHANG Xu，XU Jian，LI Ji，LOU Qunfeng，and CHEN Jinfeng*. QTL Mapping and Identification of Candidate Gene for Resistance to Gummy Stem Blight in Cucumis sativus/hystrix Introgression Line‘IL77’[J]. ACTA HORTICULTURAE SINICA, 2018, 45(11): 2141-2152.

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QTL Mapping and Identification of Candidate Gene for Resistance to Gummy Stem Blight in Cucumis sativus/hystrix Introgression Line‘IL77’

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