Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 304-318.doi: 10.16420/j.issn.0513-353x.2021-0044

• Research Papers • Previous Articles     Next Articles

Identification of LEA Family and Expression Analysis of Some Members Under Low-temperature Stress in Chinese Cabbage

WANG Guangpeng, LIU Tongkun, XU Xinfeng, LI Zhubo, GAO Zhanyuan, HOU Xilin()   

  1. State Key Laboratory of Crop Genetics & Germplasm Enhancement,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China),Ministry of Agriculture and Rural Affairs,Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops,Ministry of Education,Nanjing 210095,China
  • Received:2021-04-06 Revised:2021-10-14 Online:2022-02-25 Published:2022-02-28
  • Contact: HOU Xilin


This study was based on the whole genome data of Chinese cabbage to identify and bioinformatics the late embryogenesis abundant protein(LEA)family genes. The sequence characteristics,gene structure,promoter cis-acting elements,chromosome localization,evolutionary relationships,and expression patterns in different tissues and under low temperature stress were investigated. The results showed that 65 family members were identified from the Chinese cabbage genome and could be divided into eight groups according to phylogenetic and conserved motif analysis. Fewer introns are prevalent and unevenly distributed on ten chromosomes,with three other members located on the Scaffold. The promoter cis-acting element analysis showed that 29 members(44.6%)had low temperature response elements. In addition,the proportion of light response elements and hormone response elements was the highest. The collinearity analysis showed that BrLEAs and AtLEAs had higher homology and were conservative in evolution. Analysis of transcriptome data of different tissues of Chinese cabbage found that the expression of BrLEA genes in different tissues was different and had tissue specificity. The expression of 13 family members under low temperature stress family members showed that the expression of low temperature stress increased.

Key words: Chinese cabbage, gene family of LEA, low temperature stress, bioinformatics

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