关键词: 岷江百合, lilyABC1基因, 基因克隆, 表达特性, 非生物胁迫

Abstract: LilyABC1，a gene encoding protein kinase，was cloned and its expression patterns in Lilium regale Wilson under abiotic stresses were dissected for the purpose of providing a candidate gene for lily breeding for resistance to various abiotic stresses. Plant material of wild Lilium regale Wilson was used to clone the full-length cDNA sequence of lilyABC1 using RACE technology in combination with cDNA library. A number of appropriate bioinformatics softwares were used to assay the structure of cDNA sequence and the function of deduced lilyABC1. The real-time quantitative PCR（qRT-PCR）method was applied to determine lilyABC1 expression patterns in specific tissues under different abiotic stresses including PEG-6000，NaCl，low temperature，high temperature and darkness. The results showed that thelilyABC1 cDNA was 2 333 bp with a 2 007 bp open readings frame in full-length. The sequence of the lilyABC1 consists of 668 amino acids with a molecular mass of 74.84 kD. The pI value of this protein is 5.46 and containing STYKc conserved domain. The expression levels of lilyABC1 were ranked as leaf > flower bud > bulbs；There was no obvious expression difference among different development stages of the flower buds. The expression of LilyABC1 was up-regulated by NaCl，low temperature，high temperature and darkness，but it was not sensitive to the PEG-6000. Therefore，lilyABC1 might have contributed to its resistance to abiotic stresses such as NaCl，low temperature，high temperature，dark and drought.

Key words: Lilium regale, lilyABC1, gene clone, expression, abiotic stress