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园艺学报 ›› 2021, Vol. 48 ›› Issue (9): 1706-1716.doi: 10.16420/j.issn.0513-353x.2020-0961

• 研究论文 • 上一篇    下一篇

大蒜生物钟基因AsRVE1AsRVE2及其在渗透胁迫下的表达分析

卞诗村1, 陆雅妮1, 许吴俊1, 陈伯清2, 王广龙2,*(), 熊爱生3,*()   

  1. 1淮阴工学院应用技术学院,江苏淮安 223003
    2淮阴工学院生命科学与食品工程学院,江苏淮安 223003
    3南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,农业农村部华东地区园艺作物生物学与种质创制重点实验室,南京 210095
  • 收稿日期:2021-05-13 修回日期:2021-07-31 出版日期:2021-09-25 发布日期:2021-09-30
  • 通讯作者: 王广龙,熊爱生 E-mail:guanglongwang@hyit.edu.cn;xiongaisheng@njau.edu.cn
  • 基金资助:
    江苏省杰出青年基金项目(BK20130027);教育部新世纪优秀人才支持计划项目(NCET-11-0670);江苏省高等学校大学生创新创业训练计划项目(201911049022Y)

Garlic Circadian Clock Genes AsRVE1 and AsRVE2 and Their Expression Analysis Under Osmotic Stress

BIAN Shicun1, LU Yani1, XU Wujun1, CHEN Boqing2, WANG Guanglong2,*(), XIONG Aisheng3,*()   

  1. 1Faculty of Applied Technology,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China
    2School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China
    3College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-05-13 Revised:2021-07-31 Online:2021-09-25 Published:2021-09-30
  • Contact: WANG Guanglong,XIONG Aisheng E-mail:guanglongwang@hyit.edu.cn;xiongaisheng@njau.edu.cn

摘要:

为了解大蒜生物钟相关基因REVEILLEsRVEs)的序列特征及其在渗透胁迫下的功能,从大蒜中克隆得到AsRVE1AsRVE2基因,并对其在盐胁迫和模拟干旱胁迫下的表达特征进行了分析。序列分析表明,AsRVE1AsRVE2分别含有1 050和975 bp的开放阅读框,编码349和324个氨基酸。在进化关系上,大蒜AsRVE和AsRVE2与玉米ZmRVE2和凤梨AcRVE2的较近。不同植物RVE氨基酸序列同源性较低,但N端保守结构域序列一致性较高。AsRVE1AsRVE2均能响应昼夜节律的变化,在大蒜不同组织中均能表达,在不同组织间AsRVE1的表达差异不大,AsRVE2在根中表达相对较高。干旱胁迫和盐胁迫在不同组织内均诱导了AsRVE1AsRVE2的表达。结果表明,AsRVE1AsRVE2可能参与了大蒜植株抵御盐胁迫和干旱胁迫的过程,可进一步鉴定其生物学功能。

关键词: 大蒜, 生物钟, RVE转录因子, 渗透胁迫, 基因克隆, 表达分析

Abstract:

In order to understand the sequence characteristics of circadian clock-related genes REVEILLEsRVEs)in garlic and their roles in osmotic stress response,AsRVE1 and AsRVE2 genes were isolated from garlic,and their expression patterns under salt stress and simulated drought stress were analyzed by qRT-PCR. Sequence analysis showed that the open reading frames of AsRVE1 and AsRVE2 genes were 1 050 and 975 bp in length,encoding 349 and 324 amino acids,respectively. In terms of evolutionary relationship,AsRVE1 and AsRVE2 were relatively close to ZmRVE2 in corn and AcRVE2 in pineapple. The RVE amino acid sequences of different plants had low homology,but showed high consistency in the conserved domain at the N-terminus. Both AsRVE1 and AsRVE2 genes can respond to changes in circadian rhythms,and their expression can be detected in different tissues;the expression of AsRVE1 in different tissues was not obviously different,the expression of AsRVE2 in the roots was relatively high. Both drought stress and salt stress induced the altered expression of AsRVE1 and AsRVE2 genes in different tissues. These results indicated that AsRVE1 and AsRVE2 genes may be involved in drought- and salt-stress response in garlic. The AsRVE1 and AsRVE2 genes cloned in this study laid a foundation for further identification of the biological functions and regulatory mechanisms of RVE.

Key words: garlic, circadian rhythm, RVE transcription factor, osmotic stress, gene cloning, expression analysis

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