园艺学报 ›› 2022, Vol. 49 ›› Issue (6): 1327-1338.doi: 10.16420/j.issn.0513-353x.2021-0468

• 研究论文 • 上一篇    下一篇


刘瑶瑶, 吴严严, 石岩, 毛天宇, 包满珠, 张俊卫, 张杰()   

  1. 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉 430070
  • 收稿日期:2021-12-27 修回日期:2022-03-30 出版日期:2022-06-25 发布日期:2022-07-05
  • 通讯作者: 张杰
  • 基金资助:

Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume

LIU Yaoyao, WU Yanyan, Shi Yan, MAO Tianyu, BAO Manzhu, ZHANG Junwei, ZHANG Jie()   

  1. Key Laboratory of Horticultural Plant Biology of Ministry of Education,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-12-27 Revised:2022-03-30 Online:2022-06-25 Published:2022-07-05
  • Contact: ZHANG Jie


在前期转录组数据的基础上对梅花(Prunus mume)垂枝候选基因PmTAC1TILLER ANGLE CONTROL 1)进行了克隆及相关分析。PmTAC1编码区全长915 bp,编码304个氨基酸,且在梅花垂枝和直枝品种中无序列差异。氨基酸序列比对进一步证实PmTAC1具备IGT家族典型结构域,进化树分析表明PmTAC1与桃(Prunus persica)的PpeTAC1亲缘关系最近,亚细胞定位预测该蛋白定位于细胞核和叶绿体,蛋白疏水性分析显示其具有亲水性。PmTAC1在茎中表达最高,叶和顶芽次之。PmTAC1在垂枝品种的1年生枝条中的表达显著高于直枝品种,且在两种枝型枝条近远轴侧均有差异。垂枝品种PmTAC1的启动子序列长度为1 608 bp,直枝品种启动子序列长度为1 379 bp。启动子顺式元件预测到光响应、赤霉素合成、脱落酸响应顺式元件的数量在两种枝型品种间具有差异。推测PmTAC1在垂枝和直枝梅花品种中的启动子序列及表达量差异可能与垂枝性状形成有关。

关键词: 梅花, 垂枝性状, PmTAC1, 基因克隆, 表达


A candidate gene PmTAC1TILLER ANGLE CONTROL 1)of the weeping trait of Prunus mume was cloned and analyzed based on our previous transcriptome data. The coding sequence of PmTAC1 was 915 bp,encoding 304 amino acids,and no differences was observed between upright and weeping cultivars of mei. The amino acid sequence alignment further confirmed that PmTAC1 had the typical domain of the IGT family,and evolutionary analysis showed that the genetic relationship between PmTAC1 and PpeTAC1 was the closest. Prediction of subcellular localization and hydrophobic analysis of protein showed that the protein was located in the nucleus and chloroplast,and was hydrophilic. The expression of PmTAC1 in branches of weeping cultivars was significantly higher than that in 1-year-old branches of upright cultivars,and there were differences in the adaxial and abaxial of the two types of branches. The promoter sequences of PmTAC1 in weeping and upright mei were 1 608 bp and 1 379 bp respectively,which were consistent among different cultivars of the same branch types of mei. Differences were detected in the number of light responses,gibberellin,and abscisic acid response cis-elements between the two promoter sequences. In conclusion,the promoter sequence and expression level of PmTAC1 in weeping and upright cultivars of mei may be related to the formation of weeping traits.

Key words: Prunus mume, weeping trait, PmTAC1, gene cloning, expression