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园艺学报 ›› 2022, Vol. 49 ›› Issue (5): 1102-1110.doi: 10.16420/j.issn.0513-353x.2021-0159

• 研究报告 • 上一篇    下一篇

外源褪黑素调控活性氧代谢诱导梨果实抗采后黑斑病

向妙莲1, 吴帆1, 李树成1, 马巧利1, 王印宝1, 肖刘华1, 陈金印1,2, 陈明1,*()   

  1. 1江西农业大学农学院,江西省果蔬采后处理关键技术与质量安全协同创新中心,江西省果蔬保鲜与无损检测重点实验室,南昌 330045
    2萍乡学院,江西萍乡 337055
  • 收稿日期:2022-01-12 修回日期:2022-02-28 出版日期:2022-05-25 发布日期:2022-05-25
  • 通讯作者: 陈明 E-mail:mingchen@jxau.edu.cn
  • 基金资助:
    江西省果蔬采后处理关键技术及质量安全协同创新中心项目(JXGS-03);江西省教育厅研究项目(GJJ160355);江西省教育厅青年科学基金项目项目(GJJ13255)

Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot

XIANG Miaolian1, WU Fan1, LI Shucheng1, MA Qiaoli1, WANG Yinbao1, XIAO Liuhua1, CHEN Jinyin1,2, CHEN Ming1,*()   

  1. 1Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Jiangxi Key Laboratory for Postharvest Technology and Non-destructive Testing of Fruits & Vegetables,College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China
    2Pingxiang University,Pingxiang,Jiangxi 337055,China
  • Received:2022-01-12 Revised:2022-02-28 Online:2022-05-25 Published:2022-05-25
  • Contact: CHEN Ming E-mail:mingchen@jxau.edu.cn

摘要:

为探究外源褪黑素(Melatonine,MT)诱导梨果实抗采后黑斑病的作用,以‘翠冠’梨为材料,测定MT对梨黑斑病菌(Alternaria alternata)的抑菌作用和诱导果实抗黑斑病的效应。结果表明,0.025 ~ 0.300 mmol · L-1 MT处理对A. alternata菌丝生长和孢子萌发均无明显抑制作用,但MT处理梨果实可显著降低黑斑病发生,其中0.100 mmol · L-1 MT诱导梨抗黑斑病的效应最高可达45.04%。与对照相比,0.100 mmol · L-1 MT处理显著提高了梨果实过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和多酚氧化酶(PPO)活性和总酚含量,有效降低丙二醛(MDA)积累,暗示MT处理诱导梨果实对黑斑病的抗性可能与其调控活性氧代谢有关。

关键词: 梨, 果实, 黑斑病, Alternaria alternata, 褪黑素, 诱导抗病, 活性氧代谢

Abstract:

‘Cuiguan’pears were used as materials to explore the effect of exogenous melatonin(MT)on the induced resistance to postharvest pear black spot caused by Alternaria alternata. To this end,the resistance to A. alternata and black spot were detected in MT treated pear fruits. The results showed that MT treatment(0.025-0.300 mmol · L-1)had no significant inhibition on mycelial growth and spore germination rate of A. alternata. However,it could remarkably reduce black spot of pear fruit and 0.100 mmol · L-1 MT treatment had the highest induced resistance effect of 45.04%. In comparison with the control,the activities of CAT,SOD,POD,APX and PPO,as well as the content of total phenolics,were obviously increased in 0.100 mmol · L-1 MT treated fruits and the accumulation of MDA was just effectively reduced on the contrary. The above results indicated that exogenous melatonin inducing pear fruit resistance against A. alternata infection might be related to the regulation of reactive oxygen species metabolism.

Key words: pear, fruit, black spot, Alternaria alternata, melatonin, induced resistance, reactive oxygen metabolism

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