63份柚类资源S基因型鉴定

doi:10.16420/j.issn.0513-353x.2021-0260

园艺学报 ›› 2022, Vol. 49 ›› Issue (5): 1111-1120.doi: 10.16420/j.issn.0513-353x.2021-0260

63份柚类资源S基因型鉴定

韦壮敏¹^,⁴, 魏斯佳¹, 陈鹏², 胡健兵¹, 汤雨晴³, 叶俊丽¹, 李先信², 邓秀新¹, 柴利军¹^,^**()

¹华中农业大学园艺植物生物学教育部重点实验室,武汉 430070
²湖南省农业科学院园艺研究所,长沙 410125
³江西省农业科学院园艺研究所,南昌 330200
⁴广西壮族自治区亚热带作物研究所,南宁530001

收稿日期:2022-01-19 修回日期:2022-02-05 出版日期:2022-05-25 发布日期:2022-05-25
通讯作者: 柴利军 E-mail:chailijun@mail.hzau.edu.cn
基金资助:
国家重点研发计划项目(2021YFD1200200);国家自然科学基金项目(32122075);国家自然科学基金项目(32072523);华中农业大学自主科技创新基金项目(2662019PY044)

Identification of S-genotypes of 63 Pummelo Germplasm Resources

WEI Zhuangmin¹^,⁴, WEI Sijia¹, CHEN Peng², HU Jianbing¹, TANG Yuqing³, YE Junli¹, LI Xianxin², DENG Xiuxin¹, CHAI Lijun¹^,^**()

¹Key Laboratory of Horticultural Plant Biology（Ministry of Education）,Huazhong Agricultural University,Wuhan 430070,China
²Institute of Horticulture,Hunan Academy of Agricultural Sciences,Changsha 410125,China
³Institute of Horticulture,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China
⁴Guangxi Subtropical Crops Research Institute,Nanning 530001,China

Received:2022-01-19 Revised:2022-02-05 Online:2022-05-25 Published:2022-05-25
Contact: CHAI Lijun E-mail:chailijun@mail.hzau.edu.cn

摘要/Abstract

摘要：

以‘晚白柚’等63份柚类资源为材料基础,通过RNA-seq和序列特征分析发掘新的S-RNase,并结合前期已有的9个S-RNase基因信息,设计21对特异性引物,扩增鉴定63份柚类资源的S基因型。结果发掘到12个新的S-RNase,按顺序分别命名为S₁₀-RNase ~ S₂₁-RNase。特异扩增结果显示：鉴定的63份材料中,有59份材料得到完整的S基因型,4份仅鉴定到1个S-RNase基因,其中S₂-RNase扩增频率最高,达34.92%（22/63）,S₄-RNase和S₆-RNase在63份材料中均未扩增出。结果还显示许多品种具有相同的S基因型。

关键词: 柑橘, S基因型, 自交不亲和, 特异性引物

Abstract:

Based on 63 pummelo germplasm resources,new S-RNases were identified by RNA-seq and sequence analysis. Twenty-one pairs of specific primers were designed to amplify and identify the S-genotypes of 63 pummelo germplasm resources. Results showed twelve new S-RNases were found,named S₁₀-RNase-S₂₁-RNase in sequence. Specific amplification showed that 59 out of 63 materials were identified with complete S-genotype,only one S-RNase gene was identified in four materials. Among them,S₂-RNase was amplified in 22 materials with the highest frequency of 34.92%（22/63）,while S₄-RNase and S₆-RNase were not amplified in the 63 materials. The results also showed that many varieties had the same S-genotype.

Key words: Citrus, S-genotypes, self-incompatibility, specific primer

中图分类号:

S666.3

韦壮敏, 魏斯佳, 陈鹏, 胡健兵, 汤雨晴, 叶俊丽, 李先信, 邓秀新, 柴利军. 63份柚类资源S基因型鉴定[J]. 园艺学报, 2022, 49(5): 1111-1120.

WEI Zhuangmin, WEI Sijia, CHEN Peng, HU Jianbing, TANG Yuqing, YE Junli, LI Xianxin, DENG Xiuxin, CHAI Lijun. Identification of S-genotypes of 63 Pummelo Germplasm Resources[J]. Acta Horticulturae Sinica, 2022, 49(5): 1111-1120.

图/表 6

参考文献 33

[1]	Chai L J, Kumar B M, Ge X X, Deng X X. 2010. Isolation,characterization,and expression analysis of an SKP1-like gene from‘Shatian’pummelo (Citrus grandis Osbeck). Plant Molecular Biology Reporter, 28:569-577. doi: 10.1007/s11105-010-0184-2 URL
[2]	Chen Hui, Zhang Shu-jun, Zhang Yu-yan, Heng Wei, Wu Jun,Zhang Shao-ling. 2013. Identification of S-genotypes in forty pear cultivars and analysis of S-RNase genes frequency in Pyrus. Journal of Nanjing Agricultural University, 36 (5):21-26. (in Chinese)
	陈慧, 张树军, 张妤艳, 衡伟, 吴俊, 张绍铃. 2013. 40个梨品种S基因型的鉴定及S基因频率分析. 南京农业大学学报, 36 (5):21-26.
[3]	Chen S F, Zhou Y Q, Chen Y R, Gu J. 2018. fastp:an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics, 34 (17):884-890. doi: 10.1093/bioinformatics/btx692 URL
[4]	Cheng Y J, Guo W W, Yi H L, Pang X M, Deng X X. 2003. An efficient protocol for genomic DNA extraction from Citrus species. Plant Molecular Biology Reporter, 21 (2):177-178. doi: 10.1007/BF02774246 URL
[5]	de Nettancourt D. 1997. Incompatibility in angiosperms. Sexual Plant Reproduction, 10 (4):185-199. doi: 10.1007/s004970050087 URL
[6]	Ding Ti-yu, Wu Meng-meng, Zhang Rui-ping, Yan zhen-li, Yan Ya-ru, Chen Di-xin, Zhang Heng-tao, Gao Qi-ming. 2018. Identification of S-genotypes of 20 apple cultivars. Acta Horticulturae Sinica, 45 (12):2277-2290. (in Chinese)
	丁体玉, 吴萌萌, 张瑞萍, 阎振立, 闫亚茹, 陈迪新, 张恒涛, 高启明. 2018. 20个苹果品种的S基因型鉴定. 园艺学报, 45 (12):2277-2290.
[7]	Eddy S R. 2008. A probabilistic model of local sequence alignment that simplifies statistical significance estimation. PLoS Computational Biology, 4 (5):2046-2052.
[8]	Grabherr M G, Haas B J, Yassour M, Levin J Z, Thompson D A, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren B W, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A. 2011. Full-length transcriptome assembly from RNA-seq data without a reference genome. Nature Biotechnol, 29 (7):644-652. doi: 10.1038/nbt.1883 URL
[9]	Jiang Jing-dong. 2020. Evaluation of self-incompatibility of Fortunella hindsii and exploration of its tetraploid germplasm[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	蒋靖东. 2020. 山金柑自交不亲和性评价及其四倍体种质资源发掘[硕士论文]. 武汉: 华中农业大学.
[10]	Kao T H, Tsukamoto T. 2004. The molecular and genetic bases of S-RNase-Based Self-Incompatibility. Plant Cell, 16:72-83.
[11]	Kitahara K, Matsumoto S. 2002. Sequence of the S 10 cDNA from‘McIntosh’apple and a PCR-digestion identification method. HortScience, 37 (1):187-190. doi: 10.21273/HORTSCI.37.1.187 URL
[12]	Li Tian-zhong, Long Shen-shan, Li Mao-fu, Bai Song-ling, Meng Dong. 2011. Advances in research of the self-incompatibility genotypes (S-genotypes)in apple. Chinese Agricultural Science, 44 (6):1173-1183. (in Chinese)
	李天忠, 龙慎山, 李茂福, 白松龄, 孟冬. 2011. 苹果自交不亲和基因型(S基因)研究进展. 中国农业科学, 44 (6):1173-1183.
[13]	Li Yang, Liu Chun-sheng, Yu Jie, Shogo Matsumoto, Wang Kun, Liu Zhi, Li Tian-zhong. 2017. The establishment of apple S-genotype database and instruction. China Fruits, (5):5-8. (in Chinese)
	李洋, 刘春生, 于杰, 松本省吾, 王昆, 刘志, 李天忠. 2017. 苹果S基因型数据库的建立与使用. 中国果树, (5):5-8.
[14]	Liang M, Cao Z H, Zhu A D, Liu Y L, Tao M Q, Yang H Y, Xu Q, Wang S H, Liu J J, Li Y P, Chen C W, Xie Z Z, Deng C L, Ye J L, Guo W W, Xu Q, Xia R, Larkin R M, Deng X X, Bosch M, Franklin-Tong V E, Chai L J. 2020. Evolution of self-compatibility by a mutant Sm-RNase in citrus. Nature Plants, 6:131-142. doi: 10.1038/s41477-020-0597-3 pmid: 32055045
[15]	Liang M, Yang W, Su S Y, Fu L L, Yi H L, Chen C W, Deng X X, Chai L J. 2017. Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibilityof citrus. Molecular Genetics and Genomics, 292:325-341. doi: 10.1007/s00438-016-1279-8 pmid: 27933381
[16]	Liang Mei. 2019. Gene identification and evolution of self-incompatibility of Citrus[Ph. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	梁梅. 2019. 柑橘自交不亲和相关基因鉴定及其演化[博士论文]. 武汉: 华中农业大学.
[17]	Liu Y Z, Liu Q, Tao N G, Deng X X. 2006. Efficient isolation of RNA from fruit peel and pulp of ripening navel orange(Citrus sinensis Osbeck). Journal of Huazhong Agricultural University,(3):300-304.
[18]	McClure B A, Franklin-Tong V E. 2006. Gametophytic self-incompatibility:understanding the cellular mechanisms involved in“self”pollen tube inhibition. Planta, 224 (2):233-245. pmid: 16794841
[19]	Robert M W, Mathieu S, Felipe A S, Mosè M, Panagiotis I, Guennadi K, Evgenia V K, Evgeny M Z. 2017. BUSCO Applications from quality assessments to gene prediction and phylogenomics. Molecular Biology and Evolution, 35 (3):543-548. doi: 10.1093/molbev/msx319 URL
[20]	Sijacic P, Wang X, Skirpan A L, Wang Y, Dowd P E, McCubbin A G, Huang S, Kao T H. 2004. Identification of the pollen determinant of S-RNase-mediated self-incompatibility. Nature, 429:302-305. doi: 10.1038/nature02523 URL
[21]	Silva N F, Goring D R. 2001. Mechanisms of self-incompatibility in flowering plants. Cellular and Molecular Life Sciences, 58 (14):1988-2007. pmid: 11814052
[22]	Stein J C, Howlerr B, Boyes D C, Nasrallah M E, Nasrallah J B. 1991. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proceedings of the National Academy of U.S. Sciences, 88:8816-8820.
[23]	Takayama S, Isogai A. 2005. Self-incompatibility in plants. Annual Review of Plant Biology, 56:467-489. pmid: 15862104
[24]	Takayama S, Shiba H, Iwano M, Shimosato H, Che F S, Kai N, Watanabe M, Suzuki G, Hinata K, Isogai A. 2000. The pollen determinant of self-incompatibility in Brassica campestris. Proceedings of the National Academy of Sciences of the United States of America, 97:1920-1925.
[25]	Ou Shan-han, Liao Kui-fu, Xiao Yuan-hui, Mai Shi-qiu, Xu Cui-ming, Jiang Xiao-ting, Mei Zheng-min, Liao Lian-yong, Mo Jian-sheng, Liu Sheng-qiu, Wang Ming-zhao, Fu Cui-na, Mo Qiao-di, Liu Cui-yan. 2010. The selection of self-pollination pomelo variety“Guiyou 1”. Guangxi Agricultural Sciences, 41 (1):4-6. (in Chinese)
	区善汉, 廖奎富, 肖远辉, 麦适秋, 徐粹明, 江小婷, 梅正敏, 廖连勇, 莫健生, 刘升球, 王明召, 傅翠娜, 莫桥弟, 刘翠燕. 2010. 自花结果沙田柚品种“桂柚1号”选育初报. 广西农业科学, 41 (1):4-6.
[26]	Qi Kai-jie, Zhang Xiao-bin, Guo Cheng-bao, Sun Yong-ping, Wu Ju-you, Zhang Hu-ping, Zhang Shao-ling. 2016. Automatic configuration system of pear pollination varieties based on S-genotypes. Jiangsu Agricultural Sciences, 44 (1):396-398. (in Chinese)
	齐开杰, 张晓斌, 郭成宝, 孙永平, 吴巨友, 张虎平, 张绍铃. 2016. 基于S基因型的梨树授粉品种自动配置系统. 江苏农业科学, 44 (1):396-398.
[27]	Wheeler M J, de Graaf B H, Hadjiosif N, Perry R M, Poulter N S, Osman K, Vatovec S, Harper A, Franklin F C, Franklin-Tong V E. 2009. Identification of the pollen self-incompatibility determinant in Papaver rhoeas. Nature, 459:992-995. doi: 10.1038/nature08027 URL
[28]	Williams J S, Der J P, dePamphilis C W, Kao T H. 2014. Transcriptome analysis reveals the same 17 S-Locus F-Box genes in two haplotypes of the self-Incompatibility locus of Petunia inflata. Plant Cell, 26:2873-2888. doi: 10.1105/tpc.114.126920 URL
[29]	Wu G A, Terol J, Ibanez V, López-García A, Pérez-Román E, Borredá C, Domingo C, Tadeo F R, Caballero J C, Alonso R, Curk F, Du D L, Ollitrault P, Roose M L, Dopazo J, Gmitter F G, Rokhsar D S, Talon M. 2018. Genomics of the originand evolution of Citrus. Nature, 554:311-316. doi: 10.1038/nature25447 URL
[30]	Xue Miao-nan, Chen Teng-tu, Yang Ji-hua. 1995. observations on self and cross-compatibility in Shatianyou. Acta Horticulturae Sinica, 22 (2):127-132. (in Chinese)
	薛妙男, 陈腾土, 杨继华. 1995. 沙田柚自交和异交亲和性观察. 园艺学报, 22 (2):127-132.
[31]	Zhang Shao-ling, Zhou Jian-tao, Xu Yi-liu, Chen Di-xin, Xu Guo-hua, Wu Gui-fa. 2003. Semi vitro culture of pear self-incompatibility and identification of the genotypes of pear. Acta Horticulturae Sinica, 30 (6):703-706. (in Chinese)
	张绍铃, 周建涛, 徐义流, 陈迪新, 徐国华, 吴桂法. 2003. 梨花柱半离体培养法及品种自交不亲和基因型鉴定. 园艺学报, 30 (6):703-706.
[32]	Zhang S Q, Liang M, Wang N, Xu Q, Deng X X, Chai L J. 2018. Reproduction in woody perennial Citrus:an update on nucellar embryony and self-incompatibility. Plant Reproduction, 31:43-57. doi: 10.1007/s00497-018-0327-4 pmid: 29457194
[33]	Zhang Xiao-li, Aishajiang Mai-maiti, Xu Ye-ting, Deng Li, Wang Ji-xun. 2018. Present Advance of S-gene genotype and S-genotypes in pear. Acta Agriculturae Boreali-Occidentalis Sinica, 27 (8):1077-1087. (in Chinese)
	张校立, 艾沙江 · 买买提, 徐叶挺, 邓莉, 王继勋. 2018. 梨S基因与S基因型鉴定的研究进展. 西北农业学报, 27 (8):1077-1087.

材料 Sample	名称 Name	基因型Genotype
柚 Pummelo	马家柚Majia Pummelo	S₁₀S₁₆
	慈利甜柚3号 Cili Tian Pummelo 3	S₃S₁₁
	早熟高糖香柚3号 Early-maturing and high-sugar Xiang Pummelo 3	S₁₃S₁₄
	无核安江香柚 Wuhe Anjiang Xiang Pummelo	S₁₃S₁₄
	龙都早香柚 Longdu Early-maturing Xiang Pummelo	S₁₄S₁₅
	慈利砧板柚 Cili Zhenban Pummelo	S₁₄S₁₆
	大果红瓤柚 Big Red Pummelo	S₉S₁₇
	金兰柚 Jinlan Pummelo	S₉S₁₇
	石榴柚 Shiliu Pummelo	S₁₇
	慈利菠萝香柚2号 Cili Boluo Xiang Pummelo 2	S₂S₁₈
	慈利金香柚 Cili Jinxiang Pummelo	S₁₈S₁₉
	狮子头柚 Shizitou Pummelo	S₂S₂₁
葡萄柚 Grapefruit	胡柚（常山）Hu Pummelo（Changshan）	S₁₁S₂₀
柠檬 Lemon	香水柠檬 Xiangshui Lemon	S₁₂

材料 Sample	名称 Name	基因型Genotype
柚 Pummelo	马家柚Majia Pummelo	S₁₀S₁₆
	慈利甜柚3号 Cili Tian Pummelo 3	S₃S₁₁
	早熟高糖香柚3号 Early-maturing and high-sugar Xiang Pummelo 3	S₁₃S₁₄
	无核安江香柚 Wuhe Anjiang Xiang Pummelo	S₁₃S₁₄
	龙都早香柚 Longdu Early-maturing Xiang Pummelo	S₁₄S₁₅
	慈利砧板柚 Cili Zhenban Pummelo	S₁₄S₁₆
	大果红瓤柚 Big Red Pummelo	S₉S₁₇
	金兰柚 Jinlan Pummelo	S₉S₁₇
	石榴柚 Shiliu Pummelo	S₁₇
	慈利菠萝香柚2号 Cili Boluo Xiang Pummelo 2	S₂S₁₈
	慈利金香柚 Cili Jinxiang Pummelo	S₁₈S₁₉
	狮子头柚 Shizitou Pummelo	S₂S₂₁
葡萄柚 Grapefruit	胡柚（常山）Hu Pummelo（Changshan）	S₁₁S₂₀
柠檬 Lemon	香水柠檬 Xiangshui Lemon	S₁₂

引物名称 Primer name	引物序列（5′-3′） Sequence of primer	退火温度/℃ Annealing temperature
S₁	F：ATGAACATTACTTTCTTCCTCTACA;R：GTTGATCTCCGTTCTTCGTG	57
S₂	F：GACTAACCTCTTTCGCTTTGC;R：CGGATCCATGCCTTTTCTAG	59
S₃	F：TCCAACATCACCTATTGTACAGC;R：GATAACGACCATCACTAAAAACG	59
S₄	F：GTTTGTTTCCTGCATTTCCTC;R：GACCCCTGTTCTTTTTTACTGC	59
S₅	F：ATGAAGGTGGCATCCATCA;R：AATAATATCCGGCCCACAG	59
S₆	F：ATGGGGACTAATTTCCTCATTATCTTT;R：CTATATTTTAACGTATCGCGGCAA	59
S₇	F：ATCATCTCGGGCGCTGTTC;R：TCCCCACAGTTCTCGGTTTT	60
S₈	F：CATTACCTATTCTGCTGCTCA;R：CCGAGTAGGGAACATGATTG	59
S₉	F：GTTGTAACGCAAAACACTTCTG;R：CGTATGAGCATGTTAGTCTTGG	59
S₁₀	F：CTGCATTGCCTCCGGCAC;R：TCGCCATTTTAACGGATCGAGG	60
S₁₁	F：GCCGCTCCAAACTCTTTCG;R：CGCAGGGAACTTGATGTTGT	60
S₁₂	F：TGCTGCAATGGCCAACTCTT;R：CCGGGTGGGGAACTTGATG	60
S₁₃	F：TCCACCACTTTTGGCTGGTC;R：TCTGCGCAACATTCGTCTCA	61
S₁₄	F：AACGAAGGCAGCTTACCTCT;R：CACGGCAGCTCTCTCCATTA	59
S₁₅	F：CAGCCTTGCCTCGTTTGAAG;R：GCTTGATCGTCAGCGCAAAG	59
S₁₆	F：CTACATCATAAATTCTTCCGGGC;R：GTGGTTTTGGTCGTTTTCCC	61
S₁₇	F：GCTCGGGGTTCCAGAAAGT;R：ACGGTTCAGGAAACTTGATACG	61
S₁₈	F：GCAGCTCAAAACTCGTCGC;R：TAAACGGCAGGGGGAGAGAA	59
S₁₉	F：GCTCAAGCTGACCACTGTTG;R：CTACCAGCGTGGAGGGAAAATA	59
S₂₀	F：TCAGGTGCAGCAGAAAACAC;R：CTTGGCCGTCCACGCATATAA	59
S₂₁	F：TCCACCACTTTTGGCTGGTC;R：CGGGTTTGGTTCGGGAAACT	59

引物名称 Primer name	引物序列（5′-3′） Sequence of primer	退火温度/℃ Annealing temperature
S₁	F：ATGAACATTACTTTCTTCCTCTACA;R：GTTGATCTCCGTTCTTCGTG	57
S₂	F：GACTAACCTCTTTCGCTTTGC;R：CGGATCCATGCCTTTTCTAG	59
S₃	F：TCCAACATCACCTATTGTACAGC;R：GATAACGACCATCACTAAAAACG	59
S₄	F：GTTTGTTTCCTGCATTTCCTC;R：GACCCCTGTTCTTTTTTACTGC	59
S₅	F：ATGAAGGTGGCATCCATCA;R：AATAATATCCGGCCCACAG	59
S₆	F：ATGGGGACTAATTTCCTCATTATCTTT;R：CTATATTTTAACGTATCGCGGCAA	59
S₇	F：ATCATCTCGGGCGCTGTTC;R：TCCCCACAGTTCTCGGTTTT	60
S₈	F：CATTACCTATTCTGCTGCTCA;R：CCGAGTAGGGAACATGATTG	59
S₉	F：GTTGTAACGCAAAACACTTCTG;R：CGTATGAGCATGTTAGTCTTGG	59
S₁₀	F：CTGCATTGCCTCCGGCAC;R：TCGCCATTTTAACGGATCGAGG	60
S₁₁	F：GCCGCTCCAAACTCTTTCG;R：CGCAGGGAACTTGATGTTGT	60
S₁₂	F：TGCTGCAATGGCCAACTCTT;R：CCGGGTGGGGAACTTGATG	60
S₁₃	F：TCCACCACTTTTGGCTGGTC;R：TCTGCGCAACATTCGTCTCA	61
S₁₄	F：AACGAAGGCAGCTTACCTCT;R：CACGGCAGCTCTCTCCATTA	59
S₁₅	F：CAGCCTTGCCTCGTTTGAAG;R：GCTTGATCGTCAGCGCAAAG	59
S₁₆	F：CTACATCATAAATTCTTCCGGGC;R：GTGGTTTTGGTCGTTTTCCC	61
S₁₇	F：GCTCGGGGTTCCAGAAAGT;R：ACGGTTCAGGAAACTTGATACG	61
S₁₈	F：GCAGCTCAAAACTCGTCGC;R：TAAACGGCAGGGGGAGAGAA	59
S₁₉	F：GCTCAAGCTGACCACTGTTG;R：CTACCAGCGTGGAGGGAAAATA	59
S₂₀	F：TCAGGTGCAGCAGAAAACAC;R：CTTGGCCGTCCACGCATATAA	59
S₂₁	F：TCCACCACTTTTGGCTGGTC;R：CGGGTTTGGTTCGGGAAACT	59

材料 Sample	序号 No.	名称 Name	采集地 Origin	基因型 Genotype
柚	1	金沙柚6号 Jinsha Pummelo 6	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃
Pummelo	2	金沙柚4号 Jinsha Pummelo 4	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁Sx
	3	金沙柚2号 Jinsha Pummelo 2	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃
	4	金沙柚1号 Jinsha Pummelo 1	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁S₅
	5	沙田柚（南昌）Shatian Pummelo（Nanchang）	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁S₂
	6	少核沙田柚 Shaohe Shatian Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁S₂
	7	无核沙田柚 Wuhe Shatian Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁S₂
	10	江坝柚 Jiangba Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₂₁
	11	丰城红壤柚 Fengcheng Red Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃
	12	谢家柚 Xiejia Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃
	13	蔡家柚 Caijia Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁₁S₁₂
	14	美国橙柚 America Orange-Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁₃S_x
	16	鹅旦柚 Edan Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃
	17	狮子头柚 Shizitou Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₂₁
	18	红壤文旦柚 Hongrang Wendan Pummelo	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃
	19	晚白柚 Wanbai Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₂S₅
	20	菊花芯柚 Juhuaxin Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₃S₁₁
	21	早熟高糖香柚4号 Early-maturing and high-sugar Xiang Pummelo 4	湖南省怀化市 Huaihua,Hunan Province	S₈S₁₅
	22	金兰柚 Jinlan Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₉S₁₇
	23	三蔸半柚 Sandouban Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₁₂S₁₃
	24	慈利香柚 Cili Xiang Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₁S₂
	25	文旦柚 Wendan Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₈S₁₇
	26	本地变异柚 Native Mutational Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₉S₁₇
	27	牛腿柚 Niutui Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₁₁S₂₁
	28	张家界本地红心柚 Zhangjiajie Native Red Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₁₀S₁₆
	29	强德勒红心柚 Red Chandler	湖南省农业科学院 Hunan Academy of Agricultural Sciences	S₉S₁₇
	30	慈利甜柚3号 Cili Tian Pummelo 3	湖南省张家界市 Zhangjiajie,Hunan Province	S₃S₁₁
	31	糯米柚 Nuomi Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₁₁S₁₄
	32	沙田柚-1（长沙） Shatian Pummelo 1（Changsha）	湖南省怀化市 Huaihua,Hunan Province	S₁S₂
	33	早熟高糖香柚3号 Early-maturing and high-sugar Xiangyou 3	湖南省怀化市 Huaihua,Hunan Province	S₁₃S₁₄
	34	冰糖柚 Bingtang Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₅S₁₃
	35	中秋蜜柚 Zhongqiu Mi Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₈S₉
	36	早熟高糖香柚1号 Early-maturing and high-sugar Xiangyou 1	湖南省怀化市 Huaihua,Hunan Province	S₈S₁₅
	37	永嘉早香柚 Yongjia Early-maturing xiang Pummelo	浙江省温州市 Wenzhou,Zhejiang Province	S₂S₈
	38	早熟高糖香柚2号 Early-maturing and high-sugar Xiangyou 2	湖南省怀化市 Huaihua,Hunan Province	S₈S₁₇
	39	慈利菠萝香柚2号Cili Boluo Xiang Pummelo 2	湖南省张家界市 Zhangjiajie,Hunan Province	S₂S₁₈
	40	安农1号 Annong Pummelo 1	湖南省怀化市 Huaihua,Hunan Province	S₁₃S₁₄
	41	安江无核红心柚母株 Mother plant of Anjiang Wuhe Red Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₉S₁₇
	42	白玉霜柚 Baiyushuang Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₁₄S_x
	43	大果红瓤柚 Big Red Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₉S₁₇
	44	假石榴柚 Jiashiliu Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₁₀S₁₃
	45	无核安江香柚Wuhe Anjiang Xiang Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₁₃S₁₄
	46	安江无核红心柚子代 Offspring of Anjiang Wuhe Hongxinyou	湖南省怀化市 Huaihua,Hunan Province	S₉S₁₇
材料 Sample	序号 No.	名称 Name	采集地 Origin	基因型 Genotype
	47	慈利矮晚柚 Cili Aiwan Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₂S₅
	48	安江红心柚 Anjiang Red Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₁₀S₁₆
	49	马家柚 Majia Pummelo	江西省上饶市 Shangrao,Jiangxi Province	S₁₀S₁₆
	50	安江酸柚 Anjiang Sour Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₂S₅
	51	慈利金香柚 Cili Jinxiang Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₁₈S₁₉
	52	龙都早香柚 Longdu Early-maturing Xiang Pummelo	四川省自贡市 Zigong,Sichuan Province	S₁₄S₁₅
	53	慈利甜柚1号 Cili Tian Pummelo 1	湖南省张家界市 Zhangjiajie,Hunan Province	S₁S₇
	54	慈利砧板柚 Cili Zhenban Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₁₄S₁₆
	55	沙田柚-2（长沙）Shatian Pummelo 2（Changsha）	湖南省怀化市 Huaihua,Hunan Province	S₁S₂
	56	安江香柚 Anjiang Xiang Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₉S₁₃
	57	石榴柚 Shiliu Pummelo	湖南省怀化市 Huaihua,Hunan Province	S₁₇S_x
	58	慈利菠萝香柚1号Cili Boluo Xiang Pummelo 1	湖南省张家界市 Zhangjiajie,Hunan Province	S₂S₁₈
	59	空心塔柚 Kongxinta Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₉S₁₇
	60	永定白柚 Yongding White Pummelo	湖南省张家界市 Zhangjiajie,Hunan Province	S₅S₁₀
	61	未知柚1号 Unknown Pummelo 1	湖南省农业科学院 Hunan Academy of Agricultural Sciences	S₁₃S₁₄
	62	未知柚2号 Unknown Pummelo 2	湖南省农业科学院 Hunan Academy of Agricultural Sciences	S₂S₂₁
	63	未知柚3号 Unknown Pummelo 3	湖南省怀化市 Huaihua,Hunan Province	S₉S₁₇
葡萄柚	8	胡柚（衢州）Hu Pummelo（Quzhou）	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁₁S₂₀
Grapefruit	9	胡柚（常山）Hu Pummelo（Changshan）	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₁₁S₂₀
	15	葡萄柚Grapefruit	江西省农业科学院 Jiangxi Academy of Agricultural Sciences	S₂S₃

63份柚类资源S基因型鉴定

Identification of S-genotypes of 63 Pummelo Germplasm Resources

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63份柚类资源S基因型鉴定

Identification of S-genotypes of 63 Pummelo Germplasm Resources

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