https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2022, Vol. 49 ›› Issue (4): 827-840.doi: 10.16420/j.issn.0513-353x.2020-1069

• 研究论文 • 上一篇    下一篇

菊花和菊花脑DNA甲基化相关酶基因鉴定及表达分析

王莹, 艾鹏慧, 李帅磊, 康冬茹, 李忠爱, 王子成**()   

  1. 河南大学生命科学学院,河南大学农学院,作物逆境适应与改良国家重点实验室,植物种质资源与遗传工程实验室,开封市菊花生物学重点实验室,河南开封 475004
  • 收稿日期:2021-07-16 修回日期:2021-11-15 出版日期:2022-04-25 发布日期:2022-04-24
  • 通讯作者: 王子成 E-mail:wzc@henu.edu.cn
  • 基金资助:
    国家重点研发计划项目(2018YFD1000403)

Identification and Expression Analysis of Genes Related to DNA Methylation in Chrysanthemum × morifolium and C. nankingense

WANG Ying, AI Penghui, LI Shuailei, KANG Dongru, LI Zhongai, WANG Zicheng**()   

  1. State Key Laboratory of Crop Stress Adaptation and Improvement,Laboratory of Plant Germplasm Resources and Genetic Engineering,Kaifeng Key Laboratory of Chrysanthemum Biology,School of Life Sciences,School of Agriculture,Henan University,Kaifeng,Henan 475004,China
  • Received:2021-07-16 Revised:2021-11-15 Online:2022-04-25 Published:2022-04-24
  • Contact: WANG Zicheng E-mail:wzc@henu.edu.cn

摘要:

基于传统菊花(Chrysanthemum × morifolium Ramat.)品种‘金背大红’转录组数据和菊花脑(C. nankingense)的基因组数据,通过生物信息学方法鉴定了二者的DNA甲基化相关酶基因,分析其编码蛋白结构域并构建系统发育树,还分析了基因的表达情况。从‘金背大红’中鉴定出了13个DNA甲基转移酶基因和11个去甲基化酶基因,从菊花脑中鉴定出10个DNA甲基转移酶基因和5个去甲基化酶基因。MET1同源氨基酸序列系统进化分析结果表明,菊花‘紫精灵’‘金背大红’和‘禾城星火’与甘菊[C. lavandulifolium(Fisch. ex Trautv.)Ling et Shih]处于较近的分支中,这表明他们可能有更近的演化关系,而菊花脑和菊花‘泉乡水长’与甘菊的进化关系可能较远。DNA甲基化相关酶基因在不同组织及不同菊花品种中的表达水平存在较大差异。

关键词: 菊花, DNA甲基化, DNA甲基转移酶, DNA去甲基化酶, 基因鉴定, 表达

Abstract:

In this study,based on the transcriptome data of the traditional chrysanthemum (Chrysanthemum × morifolium Ramat.)species‘Jinbeidahong’and the genome data of Chrysanthemum nankingense,the genes encoding DNA methylation-related enzyme were identified through bioinformatics from the‘Jinbeidahong’and C. nankingense,phylogenetic trees and gene expression were analyzed. Totally 13 DNA methyltransferase genes and 11 demethylase genes were identified from‘Jinbeidahong’,and 10 DNA methyltransferase genes and five demethylase genes were identified from C. nankingense. The evolutionary relationship demonstrated that C. lavandulifolium(Fisch. ex Trautv.)Ling et Shih were more closely associated with chrysanthemum‘Zijingling’‘Jinbeidahong’‘Hechengxinghuo’than C. nankingense and‘Quanxiangshuichang’. The transcripts levels of DNA methylation-related enzyme genes in multiple tissues and chrysanthemum varieties were quite different.

Key words: chrysanthemum, DNA methylation, DNA methyltransferase, DNA demethylase, gene identification, gene expression

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