园艺学报 ›› 2022, Vol. 49 ›› Issue (4): 816-826.doi: 10.16420/j.issn.0513-353x.2021-0007

• 研究论文 • 上一篇    下一篇


刘筱玮1, 夏斌1, 李子葳1, 杨宇佳1, 陈斌1, 周蕴薇2,**(), 何淼1,**()   

  1. 1.东北林业大学园林学院,哈尔滨 150040
    2.吉林农业大学园艺学院,长春 130118
  • 收稿日期:2021-11-29 修回日期:2022-03-07 出版日期:2022-04-25 发布日期:2022-04-24
  • 通讯作者: 周蕴薇,何淼;
  • 基金资助:

Overexpression of Chrysanthemum indicum miR396a Gene in Arabidopsis Enhances Its Salt Tolerance

LIU Xiaowei1, XIA Bin1, LI Ziwei1, YANG Yujia1, CHEN Bin1, ZHOU Yunwei2,**(), HE Miao1,**()   

  1. 1. College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China
    2. College of Horticulture,Jilin Agricultural University,Changchun 130118,China
  • Received:2021-11-29 Revised:2022-03-07 Online:2022-04-25 Published:2022-04-24
  • Contact: ZHOU Yunwei,HE Miao;


从野菊(Chrysanthemum indicum)中克隆了cin-miR396a前体序列及其启动子,对前体序列进行比对与进化分析,对启动子顺式作用元件进行分析,并研究了cin-miR396a前体序列对拟南芥的遗传转化及对其耐盐性的影响。结果表明,cin-MIR396a长度为145 bp,含有完整的茎环结构,与拟南芥ath-MIR396a进化关系最近。启动子区具有光信号、茉莉酸甲酯、赤霉素、水杨酸、脱落酸等多种响应元件,推测cin-MIR396a参与多种逆境胁迫下的应答及调控。在拟南芥中过表达cin-miR396a前体可以提高盐胁迫下种子发芽率以及成苗期的耐盐性,cin-miR396a在拟南芥响应盐胁迫中发挥正调控作用。

关键词: 野菊, miR396, 拟南芥, 转基因, 盐胁迫


cin-miR396a precursor sequence and its promoter were cloned from Chrysanthemum indicum;the precursor sequence alignment and evolutionary analysis were performed;the promoter was analyzed for cis-acting elements. The genetic transformation of the cin-miR396a precursor sequence to Arabidopsis thaliana was further carried out,and the salt tolerance of transgenic Arabidopsis thaliana was analyzed. The results showed that cin-MIR396a was 145 bp in length,contained an intact stem-loop structure,and was evolutionarily closest to A. thaliana ath-MIR396a. The promoter region has multiple response elements such as light signal,methyl jasmonate,gibberellin,salicylic acid,and abscisic acid,and it is hypothesized that cin-MIR396a is involved in response and regulation under a variety of adversity stresses. Overexpression of the cin-miR396a precursor in A. thaliana can improve seed germination under salt stress and salt tolerance at the adult seedling stage,and cin-miR396a plays a positive regulatory role in A. thaliana in response to salt stress.

Key words: Chrysanthemum indicum, miR396, Arabidopsis thaliana, transgenic, salt stress