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园艺学报 ›› 2014, Vol. 41 ›› Issue (6): 1175-1182.

• 研究报告 • 上一篇    下一篇

刺梨GDP-L–半乳糖磷酸酶基因的表达及其与AsA积累的关系

孙雅蕾,杨 曼,安华明*   

  1. (贵州大学农学院,贵州省果树工程技术研究中心,贵阳 550025)
  • 收稿日期:2014-01-14 出版日期:2014-06-25 发布日期:2014-06-25

Expression of GDP-L-galactose Pyrophosphatase and Its Relationship with Ascorbate Accumulation in Rosa roxburghii

SUN Ya-lei,YANG Man,and AN Hua-ming*   

  1. (Guizhou Engineering Research Center for Fruit Crops,Agricultural College,Guizhou University,Guiyang 550025,China)
  • Received:2014-01-14 Online:2014-06-25 Published:2014-06-25

摘要: 采用RT-PCR和RACE技术,从刺梨(Rosa roxburghii Tratt)果实中扩增出GDP-L–半乳糖磷酸酶(GDP-L-galactose pyrophosphatase,GGP)基因的全长cDNA序列,并分析其在不同组织和果实不同发育阶段的表达特点及其与AsA积累的关系。结果表明:刺梨GGP基因cDNA(RrGGP,GenBank 登录号:HM998753)包含1个长度为1 341 bp的开放阅读框(ORF),编码445个氨基酸;其氨基酸序列与苹果、猕猴桃等的相似性均在80%以上。RT-PCR分析表明,该基因在刺梨不同器官中的表达差异明显:在果实中的表达量最强,叶中次之,而花和茎中只能检测到极微弱的表达。在果实不同发育时期都能检测到RrGGP的表达,在发育初期(花后50 d内)表达较弱,花后60 ~ 100 d呈高水平表达,而后随着果实成熟而下调。刺梨中RrGGP的表达特点与AsA积累高度一致,意味着该基因可能是果实发育过程中AsA合成的关键基因。

关键词: 刺梨, GDP-L–半乳糖磷酸酶, 基因表达, AsA

Abstract: A full length cDNA encoding GDP-L-galactose pyrophosphatase(GGP),a key enzyme in the putative L-ascorbic acid(AsA)biosynthetic pathway of higher plants,was obtained from Rosa roxburghii Tratt fruits using RT-PCR and RACE technology. This cDNA,designated RrGGP(accession number,HM998753),contained an open reading frame(ORF)of 1 341 bp,encoding a protein of 445 amino acids. Sequence analysis indicated that its deduced amino acid showed over 80% identities with the corresponding proteins from other plant species. Semi-quantitative RT-PCR showed that the expression of RrGGP in fruits was significantly abundant than that of in leaves,flowers or stems,respectively,contributing to parallel accumulation of AsA. Transcript abundance of RrGGP in developing fruits showed a development-specific profile,in which RrGGP transcript levels were lower in young(10–50 days after anthesis,DAA)and nearly ripe(110–120 DAA)fruits,and higher in fruit in mid-development(60–100 DAA). This expression pattern showed a well correlation with AsA level,suggesting an important role of this gene in regulating AsA biosynthesis in the fruits.

Key words: Rosa roxburghii, GGP, gene expression, ascorbic acid

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