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园艺学报 ›› 2018, Vol. 45 ›› Issue (3): 421-435.doi: 10.16420/j.issn.0513-353x.2017-0474

• 研究论文 • 上一篇    下一篇

梨蔗糖合成相关酶SUS和SPS基因家族的鉴定与表达分析

吕佳红,王英珍,程 瑞,王国明,张绍铃,吴 俊,张虎平*   

  1. (南京农业大学园艺学院,梨工程技术研究中心,南京 210095)
  • 出版日期:2018-03-25 发布日期:2018-03-25

Genome-wide Identification and Expression Analysis of Sucrose Synthase (SUS)and Sucrose Phosphate Synthase(SPS)Gene Families in Pear

Lü Jiahong,WANG Yingzhen,CHENG Rui,WANG Guoming,ZHANG Shaoling,WU Jun,and ZHANG Huping*   

  1. (Department of Horticulture,Centre of Pear Engineering Technology Research,Nanjing Agricultural University,Nanjing 210095,China)
  • Online:2018-03-25 Published:2018-03-25

摘要: 运用生物信息学方法,对梨蔗糖合酶(SUS)和蔗糖磷酸合成酶(SPS)基因进行全基因组鉴定与基因结构、系统发育关系、蛋白质保守功能域、染色体定位和基因进化模式分析,利用转录组数据和实时荧光定量PCR技术分析低蔗糖型‘砀山酥梨’和高蔗糖型‘翠冠’果实发育过程中SUS和SPS家族基因的表达模式。结果表明,梨中包含17个SUS基因(PbrSUS1 ~ PbrSUS17)和8个SPS基因(PbrSPS1 ~ PbrSPS8);SUS基因家族分为3个亚家族,分别为SUS1、SUSA和SUS2。梨SUS和SPS基因家族成员不均匀地分布在10条染色体上,主要通过片段复制事件进行扩张,且在进化过程中主要受纯化选择作用。PbrSPS3、PbrSPS4、PbrSPS8、PbrSUS2和PbrSUS15是调控梨果实蔗糖合成积累的重要基因。

关键词: 梨, 蔗糖合酶, 蔗糖磷酸合成酶, 基因, 生物信息学分析, 表达分析

Abstract: In this study,the sucrose synthase(SUS)and sucrose phosphate synthase(SPS)gene families were identified from pear(Pyrus bretschneideri‘Dangshan Suli’) genome database,and their gene structure,phylogenetic relationship,protein conserved domains,chromosomal locations and gene evolution pattern were analyzed by bioinformatic approaches. Transcriptome data and quantitative real-time PCR were used separately to analyze the expression patterns of SUS and SPS gene families during the fruit development of low-sucrose‘Dangshan Suli’and high-sucrose‘Cuiguan’(P. pyrifolia). The results showed 17 SUS genes(PbrSUS1–PbrSUS17)and 8 SPS genes(PbrSPS1–PbrSPS8)were identified in P. bretschneideri,including 3 subfamilies for SUS gene family(SUS1,SUSA and SUS2). The expansion of PbrSUS and PbrSPS gene families distributed unevenly on 10 chromosomes were mainly caused by segmental duplication,and their evolutionary processes were affected by purify selection. PbrSPS3,PbrSPS4,PbrSPS8,PbrSUS2,and PbrSUS15 are the important genes in regulating the synthesis and accumulation of sucrose for pear fruit.

Key words: Pyrus, sucrose synthase, sucrose phosphate synthase, gene;bioinformatics analysis, expression analysis

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