Efficient Culture and Plan t Regeneration from Cotyledon Protoplasts of ‘Cuixiu’Cucmnber

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (02): 227-228.

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Efficient Culture and Plan t Regeneration from Cotyledon Protoplasts of ‘Cuixiu’Cucmnber

Guo Dezhang¹;Yan Zhen¹;Lai Zhongxiong²，Lin Qingliang²，and Wang Jiafu²

(¹Fuzhou Institute of Agriculture of Sciences Fujian Province，Fuzhou 350014，China; ²Institute of Subtropical Fruit Trees Fujian Agricultural and Forestry University，Fuzhou 350002， China)

Received:2002-05-21 Revised:2002-09-23 Online:2003-04-25 Published:2003-04-25

Abstract

Abstract: Isolated and purified cotyledon protoplasts of cucumber(Cucummis sativust L． ‘Cuixiu’)were cultured at diferent population density ranging from 5.5×10/mL to 5.5×10⁵ /mL in mKM8p liquid medium respectively an d underwent cell division in varying degrees．Sustained cel divisions with eventual somatic colony production occurred only when the population density of protoplasts exceeded 1.0×10³/mL． the highest plating efficiency was about 54% ．Lager quantifies of embryoids diferentiated from protoplast derived ealli and then converted into planflets after succedent induction culture．Results demonstrate that the concentration of Ca²⁺has an important effect on the stability an d cel division of cucumber protoplasts．

Key words: Cucumber, Cotyledon, Protop]astsPlant regeneration

CLC Number:

S642.2

Guo Dezhang;Yan Zhen;Lai Zhongxiong;Lin Qingliang;and Wang Jiafu. Efficient Culture and Plan t Regeneration from Cotyledon Protoplasts of ‘Cuixiu’Cucmnber[J]. ACTA HORTICULTURAE SINICA, 2003, 30(02): 227-228.

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Efficient Culture and Plan t Regeneration from Cotyledon Protoplasts of ‘Cuixiu’Cucmnber

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