Abstract:

The culture of the stem-bud from crown and stump shoot of Machilus yunnanensis var． duclouxii in vitro was studied．The results showed that the less browning and high germination rate of axially bud from the stem-bud appeared on the medium of MS+BA 4mg·L^-1+NAA 0.5 mg·L^-1，and the plan tlets grew well，which was beneficial to later mutipulcation．Th e tufted buds were diferentiated on the mediunl of MS+BA 8mg·L^-1 +NAA 0.5 mg·L^-1+KT 1.0 mg·L^-1，but multiplication rate was low．On the medium of 1/2 MS + NAA 0.1 mg·L^-1，the white healthy roots were induced ．The taken parts，taken-time of explants of Machilus yunnanensis var． duclouxii and hormone in medium are main factors that cause browning an d afect the speed of propagation during breeding．

Key words: Machilus yunnanensis var． duclouxii, Culture in vitro, Rapid propagation, Hormone, Browning