Abstract: The leaf regeneration and Agrobacterium tumefaciens—mediated transformation system of cherry rootstock 98-1，Colt and Dagingye were established at the first time．The results showed that the best medium Ms supplemented with 1.0—2.0 mg/L BA，0.3—0.5 mg/L NAA，0.5 mg/L GA and 5.0—10.0 mg/L AgNO₃ promoting leaf regeneration frequency.ephysiological reaction of Agrobacterium tumefaciens and explant cell were key to transformation．Delay selection could improve resistance to Kan of transformed cell in leaves and do wel to regenemtion．Results of molecular biological assays with PCR an d Southern blot hybridization preliminarily indicated that the an tibacterial peptide genes had been integrated into the genome of cherry．

Key words: Cherry, Regeneration, Antibacterial peptide gene, Agrobacterium tumefacens-mediated transformation