Development of a Multiplex PCR Protocol for the Detection of Two Virusesof Banana

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 845-848.

• 研究报告 • Previous Articles Next Articles

Development of a Multiplex PCR Protocol for the Detection of Two Virusesof Banana

Peng Jun¹;Wang Guofen¹;Huang Junsheng^{1, 2*};Dai Peng¹;Xie Yuping¹;Li Weidong³

(¹ Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737,China; ²State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China; ³Hainan Entry-exit Inspection and Quarantine Bureau of Peoples Republic of China, Haikou, Hainan 570311, China)

Received:2005-08-16 Revised:2005-12-01 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: A multiplex RT-PCR was developed for the simultaneous detection of two viruses of banana and plantain (Musa spp. ). Two sets of specific primer pairs were designed according to the gene sequence of Banana bunchy top virus (BBTV) replicase region and Cucumber mosaic virus (CMV) coat protein region, respectively. Based upon the establishment of the optimized PCR and RT-PCR detection of BBTV and CMV, the multiplex RT-PCR which can simultaneously detect the two banana viruses was developed. It was shown that all samples infected with BBT and CMV could be amplified simultaneously by multiplex RT-PCR, and yield two specific bands of BBTV (748 bp) and CMV (557 bp) visualized by agarose gel electrophoresis. Sequence analysis of the amplified products showed high homology of BBTV (91% - 99% ) and CMV (93% - 98% )
with the sequences of BBTV and CMV in GenBank.

Key words: Banana, Banana bunchy top virus (BBTV), Cucumber mosaic virus (CMV), Virus detection, RT-PCR

Peng Jun;Wang Guofen;Huang Junsheng;;Dai Peng;Xie Yuping;Li Weidong. Development of a Multiplex PCR Protocol for the Detection of Two Virusesof Banana[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 845-848.

[1]	ZHANG Xin, QI Yanxiang, ZENG Fanyun, WANG Yanwei, XIE Peilan, XIE Yixian, and PENG Jun. Functional Analysis of Dicer-like Genes in Fusarium oxysporum f. sp. cubense Race 4 [J]. Acta Horticulturae Sinica, 2023, 50(2): 279-294.
[2]	LIANG Jiali, WU Qisong, CHEN Guangquan, ZHANG Rong, XU Chunxiang, and FENG Shujie, . Identification of the Neopestalotiopsis musae Pathogen of Banana Leaf Spot Disease [J]. Acta Horticulturae Sinica, 2023, 50(2): 410-420.
[3]	YANG Xingyu, XU Linbing, WU Yuanli, QIU Diyang, FAN Linlin, HUANG Bingzhi. Genome（ABBB）Identification of a Hybrid Banana Cultivar‘Fenza 1’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 1991-1997.
[4]	XIA Yan, HUANG Song, WU Xueli, LIU Yiqi, WANG Miaomiao, SONG Chunhui, BAI Tuanhui, SONG Shangwei, PANG Hongguang, JIAO Jian, ZHENG Xianbo. Identification and Analysis of Apple Viruses Diseases Based on Virome Sequencing Technology [J]. Acta Horticulturae Sinica, 2022, 49(7): 1415-1428.
[5]	ZHANG Qiuyue, LIU Changlai, YU Xiaojing, YANG Jiading, FENG Chaonian. Screening of Reference Genes for Differentially Expressed Genes in Pyrus betulaefolia Plant Under Salt Stress by qRT-PCR [J]. Acta Horticulturae Sinica, 2022, 49(7): 1557-1570.
[6]	GONG Mingxia, ZHAO Hu, WANG Meng, WU Xing, ZHAO Zengjing, HE Zhi, HUANG Jinmei, MENG Shengde, WANG Risheng. Identification of Viruses Infecting Peppers in Guangxi by Small RNA Deep Sequencing and RT-PCR [J]. Acta Horticulturae Sinica, 2022, 49(5): 1060-1072.
[7]	WANG Hong, YANG Wangli, LIN Jing, YANG Qingsong, LI Xiaogang, SHENG Baolong, CHANG Youhong. Comparative Metabolic and Transcriptomic Analysis of Ripening Fruit in Pear Cultivars of‘Sucui 1’‘Cuiguan’and‘Huasu’ [J]. Acta Horticulturae Sinica, 2022, 49(3): 493-508.
[8]	LI Shifan, SHAO Yuchun, QI Haonan, YANG Lan, LI Xiaowei, XU Bingliang, CHEN Yahan. Detection and Genetic Diversity Analysis of Apple Necrotic Mosaic Virus in Gansu Province [J]. Acta Horticulturae Sinica, 2022, 49(11): 2431-2438.
[9]	MENG Zhen, ZHANG Weiping, WANG Ying, LI Long, JI Xiaoxue, DONG Bei, QIAO Kang. Establishment and Application of Real-Time PCR for Quantitative Detection of Fusarium oxysporum f. sp. lycopersici [J]. Acta Horticulturae Sinica, 2022, 49(11): 2479-2488.
[10]	ZHANG Zhiqiang, LU Shixiong, MA Zonghuan, LI Yanbiao, GAO Caixia, CHEN Baihong, MAO Juan. Bioinformatic Identification and Expression Analysis of Candidate Genes of LIM Protein Family in Strawberry Under Abiotic Stresses [J]. Acta Horticulturae Sinica, 2021, 48(8): 1485-1503.
[11]	ZHU Junfei, LI Xin, DONG Kangting, TANG Zhifei, BIAN Xiuju, WANG Lihong, LI Huibin, SUN Xinbo. Effect of Creeping Bentgrass Small Heat Shock Protein AsHSP26.8a on Transgenic Photosynthesis in Arabidopsis thaliana [J]. Acta Horticulturae Sinica, 2021, 48(8): 1619-1625.
[12]	YANG Fei, ZHANG Aihong, WANG Xiuzhi, LI Xiwang, DI Dianping, MIAO Hongqin, ZHANG Shangqing, SUN Xiangrui, SUN Qian. Identification of Tomato spotted wilt orthotospovirus in Chifeng,Inner Mongolia [J]. Acta Horticulturae Sinica, 2021, 48(6): 1208-1216.
[13]	HUANG Weijian, LI Meng. Status and Prospects Whole Genome Sequencing in Fruit Trees [J]. Acta Horticulturae Sinica, 2021, 48(4): 733-748.
[14]	JIA Bing, GUO Guoling, WANG Youyu, YE Zhenfeng, LIU Li, LIU Pu, HENG Wei, ZHU Liwu. Studies on the Regreening Mechanism of the Iron-deficiency Chlorosis Leaves induced by GA₃ in‘Whangkeumbae’（Pyrus pyrifolia Nakai） [J]. Acta Horticulturae Sinica, 2021, 48(2): 254-264.
[15]	FAN Xudong, DONG Yafeng, ZHANG Zunping, REN Fang, HU Guojun, ZHANG Mengyan, LI Chen. Detection and Sequence Analyses of Grapevine Leafroll-associated Virus 7 Isolates in China [J]. Acta Horticulturae Sinica, 2021, 48(2): 347-354.

Development of a Multiplex PCR Protocol for the Detection of Two Virusesof Banana

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments