Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 410-420.doi: 10.16420/j.issn.0513-353x.2021-1115

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Identification of the Neopestalotiopsis musae Pathogen of Banana Leaf Spot Disease

LIANG Jiali1, WU Qisong1, CHEN Guangquan2, ZHANG Rong1,3, XU Chunxiang1, FENG Shujie1,3,*()   

  1. 1. College of Horticulture,South China Agricultural University,Guangzhou 510642,China
    2. Maoming Agricultural Technology Extension Center,Maoming,Guangdong 525000,China
    3. Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Integrative Microbiology Research Centre,South China Agricultural University,Guangzhou 510642,China
  • Received:2022-08-03 Revised:2022-10-14 Online:2023-02-25 Published:2023-03-06
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A new emerging banana leaf spot disease was found during a disease survey in the banana-producing area of Maoming City,Guangdong Province. The pathogen was isolated and tested for pathogenicity according to Koch's postulate,and the pathogen was identified based on morphological characteristics and sequence analyses of ITS,β-tubulin and tef1 genes. The results showed that strain MM3-2z9 could infect banana leaves and induce similar symptoms as in the field. The colony of strain MM3-2z9 on the PDA medium was white,producing obvious concentric ring patterns. The conidia were fusoid with 4-septate,three median cells versicoloured,apical cell hyaline,pyramidal,with two to four filiform apical appendages(mostly three),and basal cell with appendage single was centric and hyaline. Sequence homology analysis indicated that the ITS,β-tubulin and tef1 sequences of three single-conidial strains of MM3-2z9 were more than 98% identical to the corresponding sequences of Neopestalotiopsis musae stains. The phylogenetic tree constructed by the three fragments showed that strain MM3-2z9 was most closely related to N. musae. Therefore,strain MM3-2z9 was identified as N. musae based on morphological analysis and phylogenetic tree analysis. In addition to damaging banana(Musa AAA Cavendish)leaves,the pathogen could also quickly infect the leaves of M. × paradisiaca ABB and Musa ABB Pisang Awak‘Fenza 1’.

Key words: banana, leaf spot disease, pathogen identification, Neopestalotiopsis musae

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